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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase BIG BROTHER (BB)

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase BIG BROTHER (BB)

  • 货号：
    CSB-YP813148DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP813148DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP813148DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP813148DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP813148DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BB
  • Uniprot No.：
  • 别名：
    BB; EOD1; At3g63530; TEL3S.1E3 ubiquitin-protein ligase BIG BROTHER; EC 2.3.2.27; Protein ENHANCER OF DA1-1; RING-type E3 ubiquitin transferase BIG BROTHER
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-248
  • 氨基酸序列
    MNGDNRPVED AHYTETGFPY AATGSYMDFY GGAAQGPLNY DHAATMHPQD NLYWTMNTNA YKFGFSGSDN ASFYGSYDMN DHLSRMSIGR TNWDYHPMVN VADDPENTVA RSVQIGDTDE HSEAEECIAN EHDPDSPQVS WQDDIDPDTM TYEELVELGE AVGTESRGLS QELIETLPTK KYKFGSIFSR KRAGERCVIC QLKYKIGERQ MNLPCKHVYH SECISKWLSI NKVCPVCNSE VFGEPSIH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that limits organ size, and possibly seed size, in a dose-dependent manner. Negatively regulates the duration of cell proliferation in leaves and petals independently of the major phytohormones (e.g. auxin, cytokinin, gibberellin, brassinosteroids, ethylene, abscisic acid, jasmonic acid), probably by targeting growth stimulators for degradation. Limits the proliferation of root meristematic cells. Polyubiquitinates DA1. Involved in the promotion of leaf senescence, in addition to its function in restricting plant growth. Possesses E3 ubiquitin-protein ligase activity in vitro.
  • 基因功能参考文献：
    1. BB acts conceptually similarly in leaf(-like) organs and the primary root, and uncouples cell proliferation from elongation in the root meristem. PMID: 28922745
    2. Big brother protein role in controlling leaf growth and senescence PMID: 28003326
    3. Genetic analyses show that DA2 functions synergistically with DA1 to regulate seed size, but does so independently of EOD1. Further results reveal that DA2 interacts physically with DA1 in vitro and in vivo. PMID: 24045020
    4. Conserved cis-elements are important for regulation of BB expression. PMID: 22433627
    5. The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner. PMID: 16461280

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  • 组织特异性：
    Mostly expressed in inflorescence, and, to a lower extent, in seedlings, roots, stems, leaves and siliques.
  • 数据库链接：

    KEGG: ath:AT3G63530

    STRING: 3702.AT3G63530.1

    UniGene: At.44049