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  4. Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase PUB22 (PUB22)

Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase PUB22 (PUB22)

  • 中文名称：
    拟南芥PUB22重组蛋白
  • 货号：
    CSB-YP890455DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PUB22重组蛋白
  • 货号：
    CSB-EP890455DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PUB22重组蛋白
  • 货号：
    CSB-EP890455DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PUB22重组蛋白
  • 货号：
    CSB-BP890455DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PUB22重组蛋白
  • 货号：
    CSB-MP890455DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PUB22
  • Uniprot No.：
  • 别名：
    PUB22; At3g52450; F22O6.170; E3 ubiquitin-protein ligase PUB22; EC 2.3.2.27; Plant U-box protein 22; RING-type E3 ubiquitin transferase PUB22; U-box domain-containing protein 22
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-435
  • 氨基酸序列
    MDQEIEIPSF FLCPISLDIM KDPVIVSTGI TYDRESIEKW LFSGKKNSCP VTKQVITETD LTPNHTLRRL IQSWCTLNAS YGIERIPTPK PPICKSEIEK LIKESSSSHL NQVKCLKRLR QIVSENTTNK RCLEAAEVPE FLANIVSNSV DTYNSPSSSL SSSNLNDMCQ SNMLENRFDS SRSLMDEALS VLYHLDTSET ALKSLLNNKK GTNLVKTLTK IMQRGIYESR AYAALLLKKL LEVADPMQII LLERELFGEV IQILHDQISH KATRSAMQIL VITCPWGRNR HKAVEGGTIS MIIELLMDDT FSSERRNSEM AMVVLDMLCQ CAEGRAEFLN HGAAIAVVSK KILRVSQITS ERAVRVLLSV GRFCATPSLL QEMLQLGVVA KLCLVLQVSC GNKTKEKAKE LLKLHARVWR ESPCVPRNLY DSYPA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that negatively regulates water stress response. May control in coordination with PUB23 a drought signaling pathway by ubiquitinating cytosolic RPN12a. Acts as negative regulator of the immunity triggered by the pathogen-associated molecular patterns (PAMPs), in association with PUB23 and PUB24. Regulates EXO70B2 ubiquitination and degradation via the 26S proteasome to attenuate PAMP-induced signaling.
  • 基因功能参考文献：
    1. PUB22-mediated degradation of Exo70B2 contributes to the attenuation of PAMP-induced signaling. PMID: 23170036
    2. The results suggest that PUB22 coordinately control a drought signaling pathway by ubiquitinating cytosolic RPN12a in Arabidopsis. PMID: 18664614
    3. Negative regulation of PAMP-triggered immunity by an E3 ubiquitin ligase triplet (PUB22, PUB23, and PUB24) in Arabidopsis. [PUB22] PMID: 18771922
  • 亚细胞定位：
    Cytoplasm.
  • 数据库链接：

    KEGG: ath:AT3G52450

    STRING: 3702.AT3G52450.1

    UniGene: At.35294