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  4. Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor WRI1 (WRI1)

Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor WRI1 (WRI1)

  • 中文名称：
    拟南芥WRI1重组蛋白
  • 货号：
    CSB-YP740965DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥WRI1重组蛋白
  • 货号：
    CSB-EP740965DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥WRI1重组蛋白
  • 货号：
    CSB-EP740965DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥WRI1重组蛋白
  • 货号：
    CSB-BP740965DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥WRI1重组蛋白
  • 货号：
    CSB-MP740965DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    WRI1
  • Uniprot No.：
  • 别名：
    WRI1; ASML1; At3g54320; T12E18.10; T12E18.20; Ethylene-responsive transcription factor WRI1; Protein ACTIVATOR OF SPORAMIN::LUC 1; Protein WRINKLED 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-430
  • 氨基酸序列
    MKKRLTTSTC SSSPSSSVSS STTTSSPIQS EAPRPKRAKR AKKSSPSGDK SHNPTSPAST RRSSIYRGVT RHRWTGRFEA HLWDKSSWNS IQNKKGKQVY LGAYDSEEAA AHTYDLAALK YWGPDTILNF PAETYTKELE EMQRVTKEEY LASLRRQSSG FSRGVSKYRG VARHHHNGRW EARIGRVFGN KYLYLGTYNT QEEAAAAYDM AAIEYRGANA VTNFDISNYI DRLKKKGVFP FPVNQANHQE GILVEAKQEV ETREAKEEPR EEVKQQYVEE PPQEEEEKEE EKAEQQEAEI VGYSEEAAVV NCCIDSSTIM EMDRCGDNNE LAWNFCMMDT GFSPFLTDQN LANENPIEYP ELFNELAFED NIDFMFDDGK HECLNLENLD CCVVGRESPP SSSSPLSCLS TDSASSTTTT TTSVSCNYLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways. Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition.
  • 基因功能参考文献：
    1. MED15 is a target of WRI1. PMID: 27246098
    2. WRINKLED1 transcription factor role in the auxin homeostasis in roots PMID: 28981783
    3. WRI1 and WRI1-regulated genes involved in fatty acid synthesis were up-regulated, providing for a corresponding increase in the rate of fatty acid synthesis. PMID: 27208047
    4. The present study describes a novel metabolic engineering ploy involving the constitutive down-regulation of endogenous ADP-glucose pyrophosphorylase (BjAGPase) enzyme and the seed-specific expression of WRINKLED1 transcription factor (AtWRI1) from Arabidopsis thaliana in Indian mustard. PMID: 27314514
    5. A C-terminal intrinsically disordered region of WRINKLED1 is important for protein stability.WRINKLED1 plays role in oil accumulation. PMID: 26305482
    6. the structure and function of the WRI1 plant transcription factor, was investigated. PMID: 23922666
    7. Data show that CUL3 and BPM proteins assemble in planta with WRI1. PMID: 23792371
    8. WRI1 activates fatty acid biosynthesis in seeds for triacylglycerol production. WRI1, WRI3 and WRI4 are required in floral tissues to provide acyl chains for cutin synthesis and prevent adherence of these developing organs and subsequent semisterility. PMID: 23243127
    9. directly controls AtGLB1 expression PMID: 21070409
    10. WRI1 is able to regulate in planta the activity of the BCCP2 and PKp-beta1 promoters to enhance the transcription level of glycolytic and fatty acid biosynthetic genes. PMID: 19719479
    11. WRI1 promotes the flow of carbon to oil during seed maturation by directly activating genes involved in fatty acid synthesis and controlling genes for assembly and storage of triacylglycerol. PMID: 19594710
    12. The putative AP2/EREBP transcription factor WRINKLED1 (WRI1) is involved in the regulation of seed storage metabolism in Arabidopsis. [WRI1] PMID: 15500472
    13. ASML1/WRI1 is a transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds [ASML1][WRI1] [WRINKLED1] PMID: 15753106
    14. WRI1 is a target of LEAFY COTYLEDON2 and is necessary for the regulatory action on fatty acid metabolism. PMID: 17419836

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    AP2/ERF transcription factor family, AP2 subfamily
  • 组织特异性：
    Mostly expressed in siliques, especially in seeds. Also detected in roots and flowers, and, to a lower extent, in leaves stems and seedlings.
  • 数据库链接：

    KEGG: ath:AT3G54320

    STRING: 3702.AT3G54320.1

    UniGene: At.35116