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  4. Recombinant Arabidopsis thaliana Gibberellin 20 oxidase 1 (20ox1)

Recombinant Arabidopsis thaliana Gibberellin 20 oxidase 1 (20ox1)

  • 中文名称：
    拟南芥GA20OX1重组蛋白
  • 货号：
    CSB-YP657879DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥GA20OX1重组蛋白
  • 货号：
    CSB-EP657879DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥GA20OX1重组蛋白
  • 货号：
    CSB-EP657879DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥GA20OX1重组蛋白
  • 货号：
    CSB-BP657879DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥GA20OX1重组蛋白
  • 货号：
    CSB-MP657879DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GA20OX1
  • Uniprot No.：
  • 别名：
    GA20OX1; 20ox1; At2301; GA5; At4g25420; T30C3.90; Gibberellin 20 oxidase 1; EC 1.14.11.-; GA 20-oxidase 1; AtGA20ox1; Gibberellin C-20 oxidase 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-377
  • 氨基酸序列
    MAVSFVTTSP EEEDKPKLGL GNIQTPLIFN PSMLNLQANI PNQFIWPDDE KPSINVLELD VPLIDLQNLL SDPSSTLDAS RLISEACKKH GFFLVVNHGI SEELISDAHE YTSRFFDMPL SEKQRVLRKS GESVGYASSF TGRFSTKLPW KETLSFRFCD DMSRSKSVQD YFCDALGHGF QPFGKVYQEY CEAMSSLSLK IMELLGLSLG VKRDYFREFF EENDSIMRLN YYPPCIKPDL TLGTGPHCDP TSLTILHQDH VNGLQVFVEN QWRSIRPNPK AFVVNIGDTF MALSNDRYKS CLHRAVVNSE SERKSLAFFL CPKKDRVVTP PRELLDSITS RRYPDFTWSM FLEFTQKHYR ADMNTLQAFS DWLTKPI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA12 and GA53 to GA9 and GA20 respectively, via a three-step oxidation at C-20 of the GA skeleton. GA53 is less effectively oxidized than GA12, and GA25 is also formed as a minor product. Involved in the promotion of the floral transition, fertility and silique elongation, but plays only a minor role in elongation of seedling organs. Acts redundantly with GA20OX2.
  • 基因功能参考文献：
    1. GA20ox1 expression affects gibberellin responses. PMID: 27879393
    2. We propose that cytokinin activity increased due to a KNOX-mediated transcriptional feedback loop resulting from the higher GNP1 transcript levels, in turn leading to increased expression of the GA catabolism genes GA2oxs and reduced GA1 and GA3 accumulation. PMID: 27764111
    3. GA20-OX1 plants accumulated more vegetative biomass than control plants in greenhouse experiments. PMID: 26903034
    4. Mutations in the early steps of the GA biosynthesis pathway led to a reduction in shoot as well as root size. Mutations at the GA5 locus yielded phenotypes characterized by decreased root length in comparison with related wild-type ones. PMID: 26162399
    5. A loss-of-function mutation due to a single nucleotide deletion in gibberellin20-oxidase1 (GA5) was identified as the cause of dwarfism in an alpine accession. PMID: 25941313
    6. antagonistic pleiotropy might underlie the occurrence of GA5 loss-of-function mutations in nature. PMID: 24023067
    7. Loss of GA20ox1, -2, and -3 blocks germination, causes severe dwarfism, delays flowering, and causes male and female sterility. PMID: 22427334
    8. Studies indicate that GA5 gene encodes a GA 20-oxidase that catalyzes the formation of the GA20 and GA9. PMID: 21103336
    9. Data show AtGA20ox1 and AtGA20ox2 act redundantly to promote hypocotyl and internode elongation, flowering time, elongation of anther filaments, the number of seeds that develop per silique and elongation of siliques. PMID: 18069939
    10. Expression of GA20ox1 is regulated at multiple levels. PMID: 18440929

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  • 蛋白家族：
    Iron/ascorbate-dependent oxidoreductase family, GA20OX subfamily
  • 组织特异性：
    Highly expressed in stems and inflorescence tissues. Detected in seeds, roots, leaves and siliques.
  • 数据库链接：

    KEGG: ath:AT4G25420

    STRING: 3702.AT4G25420.1

    UniGene: At.32258