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  4. Recombinant Arabidopsis thaliana Glycine-rich RNA-binding protein 8 (GRP8)

Recombinant Arabidopsis thaliana Glycine-rich RNA-binding protein 8 (GRP8)

  • 中文名称：
    拟南芥RBG8重组蛋白
  • 货号：
    CSB-YP311760DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RBG8重组蛋白
  • 货号：
    CSB-EP311760DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RBG8重组蛋白
  • 货号：
    CSB-EP311760DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RBG8重组蛋白
  • 货号：
    CSB-BP311760DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RBG8重组蛋白
  • 货号：
    CSB-MP311760DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RBG8
  • Uniprot No.：
  • 别名：
    RBG8; CCR1; GR-RBP8; GRP8; At4g39260; T22F8.160; Glycine-rich RNA-binding protein 8; AtGR-RBP8; AtRBG8; Glycine-rich protein 8; AtGRP8; Protein COLD; CIRCADIAN RHYTHM; AND RNA BINDING 1; Protein CCR1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-169
  • 氨基酸序列
    MSEVEYRCFV GGLAWATNDE DLQRTFSQFG DVIDSKIIND RESGRSRGFG FVTFKDEKAM RDAIEEMNGK ELDGRVITVN EAQSRGSGGG GGGRGGSGGG YRSGGGGGYS GGGGGGYSGG GGGGYERRSG GYGSGGGGGG RGYGGGGRRE GGGYGGGDGG SYGGGGGGW
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in RNA transcription or processing during stress. Binds RNAs and DNAs sequence with a preference to single-stranded nucleic acids. Involved in mRNA alternative splicing of numerous targets by modulating splice site selection. Negatively regulates the circadian oscillations of its own transcript as well as RBG7 transcript. Forms an interlocked post-transcriptional negative feedback loop with the RBG7 autoregulatory circuit. Both proteins negatively autoregulate and reciprocally crossregulate by binding to their pre-mRNAs and promoting unproductive splicing coupled to degradation via the NMD pathway. Target of the Pseudomonas syringae type III effector HopU1.
  • 基因功能参考文献：
    1. the yield penalty of ccr1 mutants was not caused by ferulic acid accumulation but was (largely) the consequence of collapsed vessels. PMID: 29158331
    2. Data show that AtGRP8 binds to AtGRP7 and levels of the alternatively spliced AtGRP8 transcripts that contain premature termination codons are strongly elevated in mutants impaired in the nonsense-mediated decay (NMD) components UPF1 or UPF3. PMID: 17924945
    3. AtGRP8 binds to its own and the AtGRP7 pre-mRNA, suggesting that this interaction is relevant for the splicing regulation. PMID: 18987006
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    GR-RBP family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: ath:AT4G39260

    STRING: 3702.AT4G39260.1

    UniGene: At.23968