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  4. Recombinant Arabidopsis thaliana Guanine nucleotide-binding protein alpha-1 subunit (GPA1)

Recombinant Arabidopsis thaliana Guanine nucleotide-binding protein alpha-1 subunit (GPA1)

  • 货号：
    CSB-YP323565DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP323565DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP323565DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP323565DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP323565DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    GPA1
  • Uniprot No.：
  • 别名：
    GPA1; At2g26300; T1D16.6; Guanine nucleotide-binding protein alpha-1 subunit; GP-alpha-1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-383
  • 氨基酸序列
    GLLCSRSRH HTEDTDENTQ AAEIERRIEQ EAKAEKHIRK LLLLGAGESG KSTIFKQIKL LFQTGFDEGE LKSYVPVIHA NVYQTIKLLH DGTKEFAQNE TDSAKYMLSS ESIAIGEKLS EIGGRLDYPR LTKDIAEGIE TLWKDPAIQE TCARGNELQV PDCTKYLMEN LKRLSDINYI PTKEDVLYAR VRTTGVVEIQ FSPVGENKKS GEVYRLFDVG GQRNERRKWI HLFEGVTAVI FCAAISEYDQ TLFEDEQKNR MMETKELFDW VLKQPCFEKT SFMLFLNKFD IFEKKVLDVP LNVCEWFRDY QPVSSGKQEI EHAYEFVKKK FEELYYQNTA PDRVDRVFKI YRTTALDQKL VKKTFKLVDE TLRRRNLLEA GLL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Exhibits a fast rate of basal nucleotide exchange. Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Together with GCR1, may regulate the cell cycle via a signaling cascade that uses phosphatidylinositol-specific phospholipase C (PI-PLC) as an effector and inositol 1,4,5-trisphosphate (IP(3)) as a second messenger. Promotes abscisic acid (ABA) responses in guard cells. Involved in the blue light (BL) signaling. Together with GCR1 and ADT3, required for BL-mediated synthesis of phenylpyruvate and subsequently of phenylalanine (Phe), in etiolated seedlings. Modulates root architecture (e.g. lateral root formation). Negatively regulated by RGS1. In collaboration with CAND2/PMTR1, regulates the melatonin-mediated stomatal closure involving H(2)O(2) and Ca(2+) signals.
  • 基因功能参考文献：
    1. Found 394 GPA1-regulated genes spanning 79 processes, including biotic and abiotic stresses, development, flavonoid biosynthesis, transcription factors, transporters and nitrate/phosphate responses. Many are unknown in published gpa1 mutant analyses. PMID: 26346778
    2. Galpha mediates ethylene-induced stomatal closure via H2O2 production, and acts downstream of RAN1, ETR1, ERS1, EIN4 and CTR1 and upstream of EIN2, EIN3 and ARR2. The data also show that ETR1 and ERS1 mediate both ethylene and H2O2 signalling in guard cells. PMID: 25704455
    3. AGG3, GPA1 and AGB1 act in the same genetic pathway to influence organ size and shape in A. thaliana. PMID: 22380792
    4. GCR1 and GPA1 are involved in N-acyl-homoserine lactones -mediated elongation of Arabidopsis roots. PMID: 22250582
    5. These findings establish a signaling pathway leading to ultraviolet-B-induced stomatal closure that involves GPA1-dependent activation of hydrogen peroxide production and subsequent Nia1-dependent nitric oxide accumulation. PMID: 23341360
    6. Both cry1 and gpa1 showed reduced accumulation of anthocyanin under blue light. Both gpa1 and cry1 mutants showed reduced GTP-binding activity. It is propsed that cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity. PMID: 22855128
    7. Absence of GPA1 interrupts abscisic acid signalling between ABA reception and reactive oxygen species production, with a consequent impairment in Ca(2+)-channel activation. PMID: 21262908
    8. Data show that the helical domain of AtGPA1 was necessary for self-activation. PMID: 21304159
    9. GPA1, AGB1, AGG and RGS1 are involved in glucose attenuation of auxin-mediated lateral root formation. PMID: 20862254
    10. A new mechanism of G-protein signaling is revealed, in which Gbeta regulates gene expression identically in the presence or absence of Galpha. PMID: 20531402
    11. GCR1 may act as a negative regulator of GPA1-mediated abscisic acid responses in guard cells PMID: 15155892
    12. The effect of extracellular calmodulin on stomatal closure in GPA1 mutant Arabidopsis. PMID: 15557100
    13. G-protein-coupled receptor 1, G-protein Galpha-subunit 1, and prephenate dehydratase 1 form all of or part of a signal transduction mechanism responsible for the light-mediated synthesis of phenylpyruvate and phenylalanine. PMID: 16415218
    14. the Gbetagamma complex serves a signaling function in the ER independent of its function in the Galphabetagamma heterotrimer PMID: 17360436
    15. This research provides evidence of the functional association of alpha and beta with the gamma subunits in Arabidopsis, while also suggesting that plasma membrane localization may be critical for function of plant heterotrimeric G proteins. PMID: 17383830
    16. may participate in pollen germination through modulation of the hyperpolarization-activated Ca(2+) channel in the plasma membrane of pollen cells PMID: 17953540
    17. These results provided the first genetic evidence that G-protein- and brassinosteroid-mediated pathways may be converged to modulate cell proliferation in a cell/tissue-specific manner. PMID: 18499742
    18. the stomatal density in Arabidopsis is modulated by GPA1 and AGB1 in an antagonistic manner. PMID: 18834874
    19. GPA1 is a positive regulator for chloroplast development in thf1 mutants. PMID: 19228339
    20. Extracellular calmodulin-mediated NO generation is regulated by GPA1, the Galpha-subunit of heterotrimeric G protein. PMID: 19321706
    21. Jasmonate-regulated gene expression is mediated by gpa1. PMID: 19342430

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  • 亚细胞定位：
    Cell membrane.
  • 蛋白家族：
    G-alpha family
  • 组织特异性：
    More abundant in roots and/or leaves.
  • 数据库链接：

    KEGG: ath:AT2G26300

    STRING: 3702.AT2G26300.1

    UniGene: At.25148