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  4. Recombinant Arabidopsis thaliana Guanine nucleotide-binding protein subunit beta-like protein A (RACK1A)

Recombinant Arabidopsis thaliana Guanine nucleotide-binding protein subunit beta-like protein A (RACK1A)

  • 货号：
    CSB-YP519570DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP519570DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP519570DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP519570DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP519570DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RACK1A
  • Uniprot No.：
  • 别名：
    RACK1A; ARCA; At1g18080; T10F20.9; T10O22.6; Receptor for activated C kinase 1A; Guanine nucleotide-binding protein subunit beta-like protein A; WD-40 repeat auxin-dependent protein ARCA
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-327
  • 氨基酸序列
    MAEGLVLKGT MRAHTDMVTA IATPIDNADI IVSASRDKSI ILWKLTKDDK AYGVAQRRLT GHSHFVEDVV LSSDGQFALS GSWDGELRLW DLAAGVSTRR FVGHTKDVLS VAFSLDNRQI VSASRDRTIK LWNTLGECKY TISEGGEGHR DWVSCVRFSP NTLQPTIVSA SWDKTVKVWN LSNCKLRSTL AGHTGYVSTV AVSPDGSLCA SGGKDGVVLL WDLAEGKKLY SLEANSVIHA LCFSPNRYWL CAATEHGIKI WDLESKSIVE DLKVDLKAEA EKADNSGPAA TKRKVIYCTS LNWSADGSTL FSGYTDGVIR VWGIGRY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Major component of the RACK1 regulatory proteins that play a role in multiple signal transduction pathways. Involved in multiple hormone responses and developmental processes. MAPK cascade scaffolding protein involved in the protease IV and ArgC signaling pathway but not the flg22 pathway.
  • 基因功能参考文献：
    1. the genes responsible for two additional suppressor mutants of acl5-1 designated sac53-d and sac56-d encode a receptor for activated C kinase 1 (RACK1) homolog and a ribosomal protein L4 (RPL4), respectively PMID: 25625317
    2. In total 97 proteins from dehydration, salt stress, ribosomal and photosynthesis pathways are found to potentially interact with RACK1A. PMID: 23435172
    3. RACK1 ensures the accumulation and processing of some miRNA precursors, directly interacts with SERRATE and is part of an AGO1 complex PMID: 23941160
    4. RACK1A protein can form complexes with the gamma subunit of the signal transducing heterotrimeric G protein. PMID: 22068106
    5. RACK1 may be required for normal production of 60S and 80S ribosomes and that its action in these processes may be regulated by abscissic acid. [RACK1A] PMID: 21098678
    6. RACK1A (At1g18080) is involved in multiple signal transduction pathways. PMID: 16829549
    7. RACK1A (At1g18080) is positively regulated by other RACK1 genes. Loss of function causes defect in plant development. PMID: 18947417
    8. RACK1 genes act redundantly to negatively regulate abscisic acid responses during seed germination and early seedling development. PMID: 19584117

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Note=Detected in the cytoplasm and nucleus when interacting with NUDT7.
  • 蛋白家族：
    WD repeat G protein beta family, Ribosomal protein RACK1 subfamily
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: ath:AT1G18080

    STRING: 3702.AT1G18080.1

    UniGene: At.22612