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  4. Recombinant Arabidopsis thaliana Histidine kinase CKI1 (CKI1), partial

Recombinant Arabidopsis thaliana Histidine kinase CKI1 (CKI1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Histidine kinase CKI1(CKI1),partial,Yeast
  • 货号：
    CSB-YP515772DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Histidine kinase CKI1(CKI1),partial,Yeast
  • 货号：
    CSB-EP515772DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Histidine kinase CKI1(CKI1),partial,Yeast
  • 货号：
    CSB-EP515772DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Histidine kinase CKI1(CKI1),partial,Yeast
  • 货号：
    CSB-BP515772DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Histidine kinase CKI1(CKI1),partial,Yeast
  • 货号：
    CSB-MP515772DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    CKI1
  • Uniprot No.：
  • 别名：
    CKI1; At2g47430; T30B22.27Histidine kinase CKI1; EC 2.7.13.3; Protein CYTOKININ-INDEPENDENT 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential protein. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Required for the development of megagametophyte in female gametophyte (embryo sac) independently of cytokinin. Contributes to vascular bundle formation and secondary growth in a cytokinin-independent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Seems to influence and promote the cytokinin signaling pathway.
  • 基因功能参考文献：
    1. Data suggest that the light-dependent regulation of CYTOKININ-INDEPENDENT 1 protein (CKI1) provides a plausible mechanistic link underlying the well-known interaction between light- and cytokinin-controlled plant development. PMID: 28292856
    2. AHP2, AHP3, and AHP5 are the major factors acting downstream of CKI1 in the two-component cytokinin signaling pathway to promote Arabidopsis female gametophyte development. PMID: 28633292
    3. The functional relevance of the conformational dynamics observed in the beta3-alpha3 loop of CKI1RD was supported by a comparison with another A. thaliana histidine kinase, ETR1. In contrast to the highly dynamic beta3-alpha3 loop of CKI1RD, the corresponding loop of the ETR1 receiver domain (ETR1RD) exhibited little conformational exchange and adopted a different orientation in crystals. PMID: 28860196
    4. Studied sequence dependence of (13) C and (15) N chemical shifts in the receiver domain of CKI1 protein from Arabidopsis thaliana, CKI1RD , and its complexed form, CKI1RD *Mg(2+), using MD/DFT calculations. MD simulations. PMID: 26879585
    5. CKI1-directed specification of the endosperm precursor central cell results in seeds containing an embryo and an endosperm. PMID: 27046830
    6. The C-terminal receiver domain of ne kinase CKI1 (CKI1(RD) ) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. PMID: 21569135
    7. CKI1-activated signaling is dependent on AHP genes which is essential for female gametophyte development. CKI1 also plays an important role during vegetative growth by regulating cell expansion. PMID: 20363773
    8. CKI-1 is involved in cytokinin perception and demonstrates the existence of a feedback loop altering cytokinin metabolism in response to the level of receptor abundance PMID: 17602786
    9. Crystals of the recombinant RD of the Arabidopsis thaliana HK CYTOKININ-INDEPENDENT1 (CKI1(RD)) have been obtained by the hanging-drop vapour-diffusion method using ammonium sulfate as a precipitant and glycerol as a cryoprotectant PMID: 19407381
    10. The results indicate that the cytokinin-independent activity of CKI1 and cytokinin-induced AHK2 and AHK3 are important for vascular bundle formation in Arabidopsis. PMID: 19622803

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 组织特异性：
    Expressed in vascular tissues of inflorescence stems and floral organs, especially in procambium cells, and in siliques.
  • 数据库链接：

    KEGG: ath:AT2G47430

    STRING: 3702.AT2G47430.1

    UniGene: At.341