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  4. Recombinant Arabidopsis thaliana MADS-box protein SVP (SVP)

Recombinant Arabidopsis thaliana MADS-box protein SVP (SVP)

  • 中文名称：
    拟南芥SVP重组蛋白
  • 货号：
    CSB-YP880830DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥SVP重组蛋白
  • 货号：
    CSB-EP880830DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥SVP重组蛋白
  • 货号：
    CSB-EP880830DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥SVP重组蛋白
  • 货号：
    CSB-BP880830DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥SVP重组蛋白
  • 货号：
    CSB-MP880830DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SVP
  • Uniprot No.：
  • 别名：
    SVP; At2g22540; F14M13.6; MADS-box protein SVP; Protein SHORT VEGETATIVE PHASE
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-240
  • 氨基酸序列
    MAREKIQIRK IDNATARQVT FSKRRRGLFK KAEELSVLCD ADVALIIFSS TGKLFEFCSS SMKEVLERHN LQSKNLEKLD QPSLELQLVE NSDHARMSKE IADKSHRLRQ MRGEELQGLD IEELQQLEKA LETGLTRVIE TKSDKIMSEI SELQKKGMQL MDENKRLRQQ GTQLTEENER LGMQICNNVH AHGGAESENA AVYEEGQSSE SITNAGNSTG APVDSESSDT SLRLGLPYGG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I am looking for the following recombinant protein:
TARGET: SVP
Expression System: E. coli
Quantity: 1mg
Tag Preference: GST-tag
Expression Region: Full-length protein
Accession#: At2g22540
Application: in vitro ubiquitination assay
Lyophilized request
Sequence:

MAREKIQIRKIDNATARQVTFSKRRRGLFKKAEELSVLCDADVALIIFSSTGKLFEFCSSSMKEVLERHNLQSKNLEKLDQPSLELQLVENSDHARMSKEIADKSHRLRQMRGEELQGLDIEELQQLEKALETGLTRVIETKSDKIMSEISELQKKGMQLMDENKRLRQQGTQLTEENERLGMQICNNVHAHGGAESENAAVYEEGQSSESITNAGNSTGAPVDSESSDTSLRLGLPYGG


If you can make this protein, please provide some informations.

A:
Thanks for your information, we have the corresponding protein, please check the details as follows:
Recombinant Arabidopsis thaliana MADS-box protein SVP(SVP)
CSB-EP880830DOA >> E.coli
You can view the product information on this page in details.
We can preferentially try GST-tag, please remark your requirement for the tag when placing the order, the success rate of providing the protein with your desired tag is 95.25%.
And we can also provide the lyophilization service for free, but lead time will be extended for 3 working days, please remark the Lyophilized request when placing the order.

靶点详情

  • 功能：
    Transcription repressor that inhibit floral transition in the autonomous flowering pathway, independent of photoperiod and temperature. Acts in a dosage-dependent manner. Together with AGL24 and AP1, controls the identity of the floral meristem and regulates expression of class B, C and E genes. Promotes EFM expression to suppress flowering.
  • 基因功能参考文献：
    1. these results reveal a novel role for the floral regulators SVP and SOC1 in disease resistance and provide evidence that salicylic acid acts directly on pathogens as an antimicrobial agent. PMID: 28812948
    2. The data suggests that AGL22 uniquely regulates a transcriptional network during drought stress, linking changes in primary metabolism and the initiation of stress responses. PMID: 26842464
    3. SVP protein up-regulates TEM2 floral repressor at low temperatures.SVP protein accumulation is higher during the day than during the night under long day conditions. PMID: 26243615
    4. Data show that microRNA miR396 triggers SHORT VEGETATIVE PHASE (SVP) mRNA decay rather than miRNA-mediated cleavage. PMID: 26103992
    5. The downstream targets of the SVP:FLC complex include a higher proportion of genes regulating floral induction, whereas those bound by either TF independently are biased towards floral development. PMID: 25853185
    6. In brm mutants, elevated PcG occupancy at SVP accompanies a dramatic increase in H3K27me3 levels at this locus and a concomitant reduction of SVP expression. PMID: 25615622
    7. post-translational regulation of SVP functions as a major mechanism for thermoregulation in flowering PMID: 24614351
    8. SVP regulates many developmental pathways, some of which are common to both of vegetative and reproductive development whereas others are specific to only one of them. PMID: 23759218
    9. The sequential formation of FUL-SVP and FUL-SOC1 heterodimers may mediate the vegetative and meristem identity transitions, counteracting the repressive effect of FLC and SVP on flowering. PMID: 24465009
    10. SVP delays flowering by repressing gibberellin biosynthesis as well as integrator gene expression. PMID: 24979809
    11. Control of SVP-FLM-beta repressor complex abundance via transcriptional and splicing regulation of FLM and posttranslational regulation of SVP protein stability provides an efficient, rapid mechanism for plants to respond to ambient temperature changes. PMID: 24030492
    12. These results support that the flowering repressor SVP has been recently selected in A. thaliana as a target for early flowering, and evidence the relevance of genetic interactions for the intraspecific evolution of FAQ1/SVP and flowering time. PMID: 23382706
    13. SVP protein directly regulates miR172 transcription in Arabidopsis PMID: 22659182
    14. Findings show that feedback regulatory loops mediated by SOC1 and SVP are essential components of the gene regulatory networks that underpin the integration of flowering signals during floral transition. PMID: 22268548
    15. LHY/CCA1 regulates a pathway negatively controlling flowering locus T (FT), possibly via ELF3-SVP/FLC. PMID: 19383102
    16. SVP negatively regulates TSF as well as FT. PMID: 19656342
    17. AGL24, AP1 and SVP directly and redundantly regulate class B, C and E floral homeotic genes. PMID: 19656343
    18. The data provides genetic evidence for the role of AP1 in these interactions by showing that the floral phenotype in the ap1 agl24 svp triple mutant is significantly enhanced. PMID: 16679456
    19. SVP mediates the temperature-dependent functions of FCA and FVE within the thermosensory pathway. PMID: 17322399
    20. once AP1 is activated during the floral transition, it acts partly as a master repressor in floral meristems by directly suppressing the expression of flowering time genes SVP, AGL24 and SOC1, preventing continuation of the shoot developmental program PMID: 17428825
    21. Complementation tests showed that rice OsMADS22 and OsMADS47 are unable to reverse the flowering-time phenotypes of the svp and agl24 mutants in Arabidopsis. PMID: 18453531
    22. AP1, AGL24 and SVP redundantly control floral meristem identity. Ectopic AGL24 and SVP expression promotes floral indeterminacy. PMID: 18694458
    23. SVP protein accumulation is regulated by LHY and CCA1. Reduction of SVP leads to accelerated flowering time. PMID: 19011118

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Detected in roots and leaves. Expressed at very low levels in flowers and siliques. Present in floral meristems.
  • 数据库链接：

    KEGG: ath:AT2G22540

    STRING: 3702.AT2G22540.1

    UniGene: At.22081