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  4. Recombinant Arabidopsis thaliana Mitogen-activated protein kinase 3 (MPK3)

Recombinant Arabidopsis thaliana Mitogen-activated protein kinase 3 (MPK3)

  • 货号：
    CSB-YP013456DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP013456DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP013456DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP013456DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP013456DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    MPK3
  • Uniprot No.：
  • 别名：
    MPK3; At3g45640; F9K21.220; T6D9.4; Mitogen-activated protein kinase 3; AtMPK3; MAP kinase 3; EC 2.7.11.24
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-370
  • 氨基酸序列
    MNTGGGQYTD FPAVETHGGQ FISYDIFGSL FEITSKYRPP IIPIGRGAYG IVCSVLDTET NELVAMKKIA NAFDNHMDAK RTLREIKLLR HLDHENIIAI RDVVPPPLRR QFSDVYISTE LMDTDLHQII RSNQSLSEEH CQYFLYQLLR GLKYIHSANI IHRDLKPSNL LLNANCDLKI CDFGLARPTS ENDFMTEYVV TRWYRAPELL LNSSDYTAAI DVWSVGCIFM ELMNRKPLFP GKDHVHQMRL LTELLGTPTE SDLGFTHNED AKRYIRQLPN FPRQPLAKLF SHVNPMAIDL VDRMLTFDPN RRITVEQALN HQYLAKLHDP NDEPICQKPF SFEFEQQPLD EEQIKEMIYQ EAIALNPTYG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking. May phosphorylate regulators of WRKY transcription factors. Mediates the phosphorylation of VIP1 and subsequent stress genes transcription in response to Agrobacterium. MKK9-MPK3/MPK6 module phosphorylates and activates EIN3, leading to the promotion of EIN3-mediated transcription in ethylene signaling. MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection. YDA-MKK4/MKK5-MPK3/MPK6 module regulates stomatal cell fate before the guard mother cell (GMC) is specified. When activated, reinforces the feedback loop by phosphorylating BASL, and inhibits stomatal fate by phosphorylating SPCH. This MAPK cascade also functions downstream of the ER receptor in regulating coordinated local cell proliferation, which shapes the morphology of plant organs.
  • 基因功能参考文献：
    1. Constitutive active-MPK3 plants are more resistant to the hemibiotrophic pathogen Pseudomonas syringae DC3000. PMID: 28766995
    2. constitutively active (CA)-MPK3 crosses with summ1 and summ2, two known suppressors of mpk4, resulted in a partial reversion of the CA-MPK3 phenotypes. PMID: 28400495
    3. Changes in PUB22 Ubiquitination Modes Triggered by MITOGEN-ACTIVATED PROTEIN KINASE3 Dampen the Immune Response PMID: 28280093
    4. MPK3 role in ultraviolet induced stomatal closure PMID: 27837091
    5. MKK3-MPK6 is activated by blue light in a MYC2-dependent manner. PMID: 25139007
    6. MicroRNA biogenesis factor DRB1 is a phosphorylation target of mitogen activated protein kinase MPK3 in both rice and Arabidopsis PMID: 25417716
    7. MPK3 represses the constitutive and flg22-induced expression of defence genes in Arabidopsis thaliana. PMID: 24980080
    8. AZI1 overexpression in the mpk3 genetic background partially alleviates the salt-hypersensitive phenotype of this mutant, but functional MPK3 appears to be required for the full extent of AZI1-conferred robustness. PMID: 24214892
    9. MYB44 is regulated by MPK3 via phosphorylation whereby initiating the adaptation response to numerous abiotic and biotic stresses. PMID: 23437396
    10. Data suggest that amino acids surrounding the phosphorylated serine residue of peptide substrates contribute to MPK3/MPK6 substrate specificity; MPK3/MPK6 activities appear to be important in growth of plant stomata. PMID: 22631074
    11. MPK3 plays role in basal and oligogalacturonide- or flagellin-induced resistance against Botrytis cinerea. PMID: 21803860
    12. MKK4 is involved in the osmotic-stress response via its regulation of MPK3 activity. PMID: 21806969
    13. These results advance understanding of the molecular mechanisms controlling AtMPK3 expression in response to different environmental stimuli. PMID: 21046323
    14. MPK3 phosphorylates ACS2 and ACS6. MPK3 and MPK6 are required for ethylene induction in Arabidopsis seedlings infected by B. cinerea. PMID: 20659280
    15. We have used ozone (O3) to generate reactive oxygen species in the apoplast of Arabidopsis thaliana and show that this treatment caused a transient activation of mitogen-activated protein kinase AtMPK3. [AtMPK3] PMID: 15500467
    16. MPK3 is a key regulator of stomatal development and patterning. PMID: 17259259
    17. These results provide clear evidence for the important role of MPK3 in the response to abscisic acid and hydrogen peroxide in guard cells. PMID: 17286820
    18. study shows VIP1 is a direct target of Agrobacterium-induced MPK3; on phosphorylation by MPK3, VIP1 relocalizes to nucleus & regulates expression of PR1; MAPK-dependent phosphorylation of VIP1 is necessary for VIP1-mediated Agrobacterium T-DNA transfer PMID: 17947581
    19. Results highlight an essential function of MPK3 in promoting cell division in the integument specifically during ovule development. PMID: 18364464
    20. the MPK3/MPK6 cascade regulates camalexin synthesis through transcriptional regulation of the biosynthetic genes after pathogen infection PMID: 18378893
    21. VIP1 binding to VIP1 response elements is enhanced under conditions of MPK3 pathway stimulation. PMID: 19820165

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cell cortex.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 数据库链接：

    KEGG: ath:AT3G45640

    STRING: 3702.AT3G45640.1

    UniGene: At.263