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  4. Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 catalytic subunit (ECR1)

Recombinant Arabidopsis thaliana NEDD8-activating enzyme E1 catalytic subunit (ECR1)

  • 中文名称：
    拟南芥ECR1重组蛋白
  • 货号：
    CSB-YP025414DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥ECR1重组蛋白
  • 货号：
    CSB-EP025414DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥ECR1重组蛋白
  • 货号：
    CSB-EP025414DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥ECR1重组蛋白
  • 货号：
    CSB-BP025414DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥ECR1重组蛋白
  • 货号：
    CSB-MP025414DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ECR1
  • Uniprot No.：
  • 别名：
    ECR1; At5g19180; T24G5_80; NEDD8-activating enzyme E1 catalytic subunit; EC 6.2.1.-; RUB-activating enzyme; Ubiquitin-activating enzyme E1-like protein
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-454
  • 氨基酸序列
    MADLDVPPQV PQSKTRDLDK LLLRHGNLVD PGFVPGPGLR DDIRDYVRIL VIGAGGLGCE LLKDLALSGF RNLEVIDMDR IEVTNLNRQF LFRIEDVGKP KAEVAAKRVM ERVSGVEIVP HFSRIEDKEI EFYNDFNIIA LGLDSIEARK YINGVACGFL EYNEDDTPKR ETIKPMVDGG TEGFKGHARV ILPGVTPCFE CTIYLFPPQV KFPLCTLAET PRNAAHCIEY AHLIQWETVH RGKTFDPDEP EHMKWVYDEA IRRAELFGIP GVTYSLTQGV VKNIIPAIAS TNAIISAACA LETLKIVSAC SKTLVNYLTY NGGEGLYTEV TKFERDTECL VCGPGILIEL DTSVTLSKFI EMLEDHPKLL LSKASVKQGE NTLYMQAPPV LEEFHRPKLS KPLYDLMGRV QKDTIHVFGQ RALKNNEKES CTTKVRVVFK GADGVADMDT AIGA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic subunit of the dimeric ECR1-AXR1 E1 enzyme. E1 activates NEDD8/RUB1 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-ECR1 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of RCE1.
  • 基因功能参考文献：
    1. AXR1 and AXL1 have similar abilities to function with E1 C-TERMINAL-RELATED1 (ECR1) in catalyzing RUB1 activation and RUB1-ECR1 thioester formation. PMID: 21311953
    2. The ecr1-1 mutant is resistant to the auxin-like compound indole-3-propionic acid, produces fewer lateral roots than wild type, displays reduced adult height, and stabilizes a reporter fusion protein that is degraded in response to auxin. PMID: 17449645
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Ubiquitin-activating E1 family, UBA3 subfamily
  • 组织特异性：
    Expressed in shoot, root and floral meristems, in vascular tissues of cotyledons and mature leaves, and in the stele of the root.
  • 数据库链接：

    KEGG: ath:AT5G19180

    STRING: 3702.AT5G19180.1

    UniGene: At.21297