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  4. Recombinant Arabidopsis thaliana Nuclear transcription factor Y subunit B-9 (NFYB9)

Recombinant Arabidopsis thaliana Nuclear transcription factor Y subunit B-9 (NFYB9)

  • 中文名称：
    拟南芥NFYB9重组蛋白
  • 货号：
    CSB-YP882844DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥NFYB9重组蛋白
  • 货号：
    CSB-EP882844DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥NFYB9重组蛋白
  • 货号：
    CSB-EP882844DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥NFYB9重组蛋白
  • 货号：
    CSB-BP882844DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥NFYB9重组蛋白
  • 货号：
    CSB-MP882844DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NFYB9
  • Uniprot No.：
  • 别名：
    NFYB9; LEC1; At1g21970; T26F17.20; Nuclear transcription factor Y subunit B-9; AtNF-YB-9; Protein LEAFY COTYLEDON 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-238
  • 氨基酸序列
    MERGAPFSHY QLPKSISELN LDQHSNNPTP MTSSVVVAGA GDKNNGIVVQ QQPPCVAREQ DQYMPIANVI RIMRKTLPSH AKISDDAKET IQECVSEYIS FVTGEANERC QREQRKTITA EDILWAMSKL GFDNYVDPLT VFINRYREIE TDRGSALRGE PPSLRQTYGG NGIGFHGPSH GLPPPGPYGY GMLDQSMVMG GGRYYQNGSS GQDESSVGGG SSSSINGMPA FDHYGQYK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the NF-Y/HAP transcription factor complex. The NF-Y complex stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters. Acts as a central regulator of the embryogenesis. Required for the speciation of cotyledon identity and the completion of embryo maturation. Controls seed storage protein genes through the regulation of FUS3 and ABI3. Involved in the blue light (BL) and abscisic acid (ABA) signaling pathways.
  • 基因功能参考文献：
    1. the mutations of key amino acids for the function of LEC1 in planta (D86K) prevented the interaction with LEC2. These results provide molecular evidences for the binding of LEC1 to B2-domain containing transcription factors, to form heteromers, involved in the control of gene expression. PMID: 29580949
    2. results show that LEC1 controls distinct gene sets at different developmental stages, including those that mediate the temporal transition between photosynthesis and chloroplast biogenesis early in seed development and seed maturation late in development. PMID: 28739919
    3. the seed-specific transcription factor LEAFY COTYLEDON1 promotes the initial establishment of an active chromatin state at FLC and activates its expression de novo in the pro-embryo, thus reversing the silenced state inherited from gametes; this active chromatin state is passed on from the pro-embryo to post-embryonic life, and leads to transmission of the embryonic memory of FLC activation to post-embryonic stages PMID: 29072296
    4. NUCLEAR FACTOR Y (NF-Y) binds a CCAAT box in the distal enhancer element and that CCAAT disruption dramatically reduces FT promoter activity PMID: 24610724
    5. In addition to VAL B3 factors, epigenetic mechanisms are implicated in maintaining repression of LEC1/AFL (LAFL)network during vegetative development PMID: 24902838
    6. Data confirms LEC1's known participation in the regulation of somatic embryogenesis, but also indicates additional roles in embryonic and extra-embryonic cell elongation PMID: 23073004
    7. NUCLEAR FACTOR Y transcription factors have both opposing and additive roles in abscisic acid-mediated seed germination. PMID: 23527203
    8. LEC1 appears to be an integrator of various regulatory events, involving the transcription factor itself as well as light and hormone signalling, especially during somatic and early zygotic embryogenesis. PMID: 22429691
    9. Cultures of the lec mutants formed somatic embryos at a low frequency. Moreover, somatic embryos were formed from callus tissue through an indirect route. PMID: 16034595
    10. A study of the network of transcription factors that regulates gene expression in A. thaliana is described and discussed. PMID: 17158584
    11. LEC1 positively regulates fatty acid biosynthesis genes. Genes involved in glycolysis and lipid accumulation are also up-regulated. PMID: 18689444
    12. LEC1 and LEC1-LIKE activate the CRC and SUCROSE SYNTHASE 2 (SUS2) promoters in combination with an NF-YC subunit through the interaction with a seed-specific ABA-response element (ABRE) binding bZIP factor, bZIP67. PMID: 19207209

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    NFYB/HAP3 subunit family
  • 组织特异性：
    Expressed in green siliques. Present in etiolated seedlings.
  • 数据库链接：

    KEGG: ath:AT1G21970

    STRING: 3702.AT1G21970.1

    UniGene: At.28011