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  4. Recombinant Arabidopsis thaliana Protein kinase PINOID (PID)

Recombinant Arabidopsis thaliana Protein kinase PINOID (PID)

  • 中文名称：
    拟南芥PID重组蛋白
  • 货号：
    CSB-YP530162DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PID重组蛋白
  • 货号：
    CSB-EP530162DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PID重组蛋白
  • 货号：
    CSB-EP530162DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PID重组蛋白
  • 货号：
    CSB-BP530162DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PID重组蛋白
  • 货号：
    CSB-MP530162DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PID
  • Uniprot No.：
  • 别名：
    PID; ABR; At2g34650; T31E10.1; Protein kinase PINOID; EC 2.7.11.1; Protein kinase ABRUPTUS
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-438
  • 氨基酸序列
    MLRESDGEMS LGTTNSPISS GTESCSSFSR LSFDAPPSTI PEEESFLSLK PHRSSDFAYA EIRRRKKQGL TFRDFRLMRR IGAGDIGTVY LCRLAGDEEE SRSSYFAMKV VDKEALALKK KMHRAEMEKT ILKMLDHPFL PTLYAEFEAS HFSCIVMEYC SGGDLHSLRH RQPHRRFSLS SARFYAAEVL VALEYLHMLG IIYRDLKPEN ILVRSDGHIM LSDFDLSLCS DSIAAVESSS SSPENQQLRS PRRFTRLARL FQRVLRSKKV QTLEPTRLFV AEPVTARSGS FVGTHEYVAP EVASGGSHGN AVDWWAFGVF LYEMIYGKTP FVAPTNDVIL RNIVKRQLSF PTDSPATMFE LHARNLISGL LNKDPTKRLG SRRGAAEVKV HPFFKGLNFA LIRTLTPPEI PSSVVKKPMK SATFSGRSSN KPAAFDYF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase involved in the regulation of auxin signaling. Acts as a positive regulator of cellular auxin efflux and regulates organ development by enhancing polar auxin transport. Phosphorylates conserved serine residues in the PIN auxin efflux carriers. Phosphorylation of PIN proteins is required and sufficient for apical-basal PIN polarity that enables directional intercellular auxin fluxes, which mediate differential growth, tissue patterning and organogenesis. Acts in association with PIN1 to control the establishment of bilateral symmetry and promotion of cotyledon outgrowth. Regulates root gravitropism through modulation of PIN2-dependent basipetal auxin transport. Required for polarization of PIN3-dependent auxin transport for hypocotyl gravitropic response. The protein kinase activity of PID is essential for its auxin efflux regulatory function. PID kinase and PP2A phosphatase activities antagonistically regulate phosphorylation of PIN proteins, affecting PIN sorting.
  • 基因功能参考文献：
    1. PID, but not D6PK, can also induce PIN1 polarity shifts, seemingly through phosphorylation at S1-S3. Phosphosite preferences are not sufficient to explain these differential effects of D6PK and PID on PIN1 polarity. PMID: 28096328
    2. CK2 also plays a key role in the transcriptional regulation of PINOID (PID), an AGC protein kinase that modulates the apical/basal localization of auxin-efflux transporters. PMID: 27275924
    3. PID is necessary for phytochrome-mediated enhancement of hypocotyl phototropism in Arabidopsis. PMID: 25281709
    4. PIN-mediated auxin transport is directly activated by D6 PROTEIN KINASE (D6PK) and PINOID (PID)/WAG kinases of the Arabidopsis AGCVIII kinase family PMID: 24948515
    5. Both PIN3 polarization and hypocotyl bending require the activity of the trafficking regulator GNOM and the protein kinase PINOID. PMID: 21569134
    6. Interaction between the PINOID/ABRUPTUS gene with the AGAMOUS gene: the negative regulator of stem cells in the meristem of Arabidopsis thaliana flower PMID: 21542343
    7. show that PID, WAG1 and WAG2, and not other plant AGC kinases, instruct recruitment of PINs into the apical recycling pathway by phosphorylating the middle serine in three conserved TPRXS(N/S) motifs within the PIN central hydrophilic loop PMID: 20823065
    8. PID regulates PIN1 polar localization through reversible phosphorylation. PMID: 20407025
    9. Interaction between the ABRUPTUS/PINOID and APETALA1 genes regulating the inflorescence development in Arabidopsis thaliana PMID: 20391782
    10. PID and GNOM antagonistically regulate PIN polarity and plant development. PID induces PIN sorting into a GNOM-independent pathway PMID: 20040538
    11. role in establishment of bilateral symmetry, as well as in the promotion of cotyledon outgrowth PMID: 15371311
    12. results show that a PINOID-dependent binary switch controls PIN polarity and mediates changes in auxin flow to create local gradients for patterning processes PMID: 15514156
    13. PDK1 is a potent enhancer of PID activity. PMID: 16601102
    14. Results suggest that PINOID positively regulates cellular auxin efflux, most likely by modulating the trafficking of PIN and/or some other molecular partners involved in auxin efflux. PMID: 16731587
    15. MAB4/ENHANCER OF PINOID is involved in polar auxin transport in organogenesis PMID: 17913786
    16. Reduced PID kinase function inhibits gravitropism and basipetal indole-3-acetic acid transport. PMID: 19363095

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  • 亚细胞定位：
    Cytoplasm, cytosol. Note=Targeted to the cell periphery.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Expressed in root hair cells, shoot xylem parenchyma cells and endodermis around the vasculature. Expressed in anther primordia, vasculature of the growing flower stalk, young pedicels and bracts and developing sepals, but not in petals. In pistils, trans
  • 数据库链接：

    KEGG: ath:AT2G34650

    STRING: 3702.AT2G34650.1

    UniGene: At.19528