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  4. Recombinant Arabidopsis thaliana Putative lipid-transfer protein DIR1 (DIR1)

Recombinant Arabidopsis thaliana Putative lipid-transfer protein DIR1 (DIR1)

  • 中文名称：
    拟南芥DIR1重组蛋白
  • 货号：
    CSB-YP840926DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥DIR1重组蛋白
  • 货号：
    CSB-EP840926DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥DIR1重组蛋白
  • 货号：
    CSB-EP840926DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥DIR1重组蛋白
  • 货号：
    CSB-BP840926DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥DIR1重组蛋白
  • 货号：
    CSB-MP840926DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DIR1
  • Uniprot No.：
  • 别名：
    DIR1; At5g48485; MJE7Putative lipid-transfer protein DIR1; Protein DEFECTIVE IN INDUCED RESISTANCE 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-102
  • 氨基酸序列
    AIDLC GMSQDELNEC KPAVSKENPT SPSQPCCTAL QHADFACLCG YKNSPWLGSF GVDPELASAL PKQCGLANAP TC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Putative lipid transfer protein required for systemic acquired resistance (SAR) long distance signaling. May interact with a lipid-derived molecule to promote long distance signaling associated with SAR. Together with AZI1, required for glycerol-3-phosphate- (G3P) and azelaic acid- (AA) induced systemic acquired resistance (SAR). Component of plant systemic immunity involved in priming defenses in a AA-dependent manner, by modulating production and/or translocation of a mobile signal(s) during SAR. Is able to bind with high affinity monoacylated phospholipids, mainly lysophosphatidylcholines.
  • 基因功能参考文献：
    1. cell-to-cell movement of DIR1 through plasmodesmata is important during long-distance systemic acquired resistance signalling in Arabidopsis. PMID: 25296648
    2. The lipid transfer proteins DIR1 which is required for G3P- and azelaic acid-induced Systemic acquired resistance, is essential for G3P accumulation. PMID: 23602565
    3. DIR1 together with a glycerol-3-phosphate-derived compound are sufficient for the induction of systemic acquired resistance in wild type plants. PMID: 22067992
    4. DIR1 contains a signal sequence that directs it to the cell wall, and is upregulated during the basal resistance response to Pseudomonas syringae. PMID: 21896186
    5. implicated in systemic acquired resistance in planta, and crystallized PMID: 16820699
    6. The structure of DIR1 reveals a new type of lipid transfer protein. PMID: 18552128

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  • 亚细胞定位：
    Secreted, extracellular space, apoplast. Endoplasmic reticulum. Cell junction, plasmodesma.
  • 蛋白家族：
    A9/FIL1 family
  • 数据库链接：

    KEGG: ath:AT5G48485

    STRING: 3702.AT5G48485.1

    UniGene: At.27209