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  4. Recombinant Arabidopsis thaliana Serine/threonine-protein kinase BIK1 (BIK1)

Recombinant Arabidopsis thaliana Serine/threonine-protein kinase BIK1 (BIK1)

  • 中文名称：
    Recombinant Arabidopsis thaliana Serine/threonine-protein kinase BIK1(BIK1),Yeast
  • 货号：
    CSB-YP528635DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Serine/threonine-protein kinase BIK1(BIK1),Yeast
  • 货号：
    CSB-EP528635DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Serine/threonine-protein kinase BIK1(BIK1),Yeast
  • 货号：
    CSB-EP528635DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Serine/threonine-protein kinase BIK1(BIK1),Yeast
  • 货号：
    CSB-BP528635DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Serine/threonine-protein kinase BIK1(BIK1),Yeast
  • 货号：
    CSB-MP528635DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    BIK1
  • Uniprot No.：
  • 别名：
    BIK1; At2g39660; F12L6.32; F17A14.3Serine/threonine-protein kinase BIK1; EC 2.7.11.1; Protein BOTRYTIS-INDUCED KINASE 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-395
  • 氨基酸序列
    GSCFSSRVK ADIFHNGKSS DLYGLSLSSR KSSSTVAAAQ KTEGEILSST PVKSFTFNEL KLATRNFRPD SVIGEGGFGC VFKGWLDEST LTPTKPGTGL VIAVKKLNQE GFQGHREWLT EINYLGQLSH PNLVKLIGYC LEDEHRLLVY EFMQKGSLEN HLFRRGAYFK PLPWFLRVNV ALDAAKGLAF LHSDPVKVIY RDIKASNILL DADYNAKLSD FGLARDGPMG DLSYVSTRVM GTYGYAAPEY MSSGHLNARS DVYSFGVLLL EILSGKRALD HNRPAKEENL VDWARPYLTS KRKVLLIVDN RLDTQYLPEE AVRMASVAVQ CLSFEPKSRP TMDQVVRALQ QLQDNLGKPS QTNPVKDTKK LGFKTGTTKS SEKRFTQKPF GRHLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in immune responses. Required to activate the resistance responses to necrotrophic pathogens, including the regulation of defense hormone expression (e.g. jasmonic acid (JA) and salicylic acid (SA)). Phosphorylates FLS2 and BAK1. Involved in pathogen-associated molecular pattern (PAMP)-triggered immunity (PTI) signaling, including calcium signaling, and defense responses downstream of FLS2; upon PAMP recognition, first phosphorylated by FLS2 prior to being monoubiquitinated by ATL44/RHA3A and ATL45/RHA3B at the plasma membrane, then internalized dynamically into endocytic compartments together with FLS2. Acts additively with PBL1 in PTI defenses. Acts as positive regulator of the PAMP flg22-induced increase of cytosolic calcium. Binds directly and phosphorylates the NADPH oxidase RBOHD at specific sites in a calcium-independent manner to enhance reactive oxygen species (ROS) generation upon flg22 perception. ROS production in response to flg22 controls stomatal movement and restriction of bacterial entry into leaf tissues. Seems not required for flg22-induced MAPK activation. Required for Pep1-induced defenses. Pep1 is an endogenous elicitor that potentiates PAMP-inducible plant responses. In association with PEPR1, acts downstream of the canonical ethylene signaling cascade to regulate ethylene responses. Involved in ethylene signaling. Destabilizes EIN3, the key transcription activator in ethylene signaling, and represses EIN3-dependent transcription. Acts as a negative regulator in brassinosteroid (BR) signaling. Functions in BR signaling by direct interaction with the BR receptor BRI1 cytosolic kinase domain.; (Microbial infection) Xanthomonas campestris effector AvrAC/XopAC-mediated uridylylation prevents activation by phosphorylation at the same residues, thus affecting immune responses and reducing defense responses toward X.campestris, mediating avrAC/XopAC virulence functions.
  • 基因功能参考文献：
    1. These findings demonstrate the mechanistic basis of signal transduction from EFR to phytohormones, mediated through a EFR-BIK1-WRKY axis. PMID: 29649442
    2. Purification, crystallization and X-ray diffraction analysis of BIK1 has been reported. PMID: 27710938
    3. Results demonstrate that BIK1 is a Pi starvation-responsive gene that functions as a negative regulator of Pi homeostasis in Arabidopsis. PMID: 27389008
    4. These data suggest that BIK1 cooperates with BAK1 to regulate constitutive immunity and cell death. PMID: 28225199
    5. Data show that Xanthomonas oryzae pv. oryzae (Xoo) PXO99A XopR protein interacts with receptor-like cytoplasmic kinase BIK1 at the plasma membrane. PMID: 27520828
    6. Upon pathogen-associated molecular patterns perception, PP2C38 is phosphorylated on serine 77 and dissociates from the FLS2/EFR-BIK1 complexes, enabling full BIK1 activation. PMID: 27494702
    7. autophosphorylated BIK1 in the plasma membrane negatively regulates EIN3-dependent gene expression. PMID: 26021844
    8. CPK28 contributes to the PAMP-induced Ca(2+) burst, supporting its role as a negative regulator of BIK1. PMID: 26039480
    9. CPK28 facilitates BIK1 turnover and negatively regulates BIK1-mediated immune responses triggered by several pathogen-associated molecular patterns PMID: 25525792
    10. required for microbe- or damage-associated molecular pattern-induced calcium signaling PMID: 25522736
    11. study first mapped the phosphorylation patterns of BAK1 associated with four RLK partners (BRI1, FLS2, EFR and BIK1 PMID: 24953020
    12. These findings highlight a direct role of the FLS2-BIK1 complex in the regulation of RbohD-mediated ROS production and stomatal defense. PMID: 24629339
    13. Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 regulates plant antibacterial immunity. PMID: 24630626
    14. The essential function of tyrosine kinase activity of BIK1 in plants echoes the function of nonreceptor tyrosine kinases that transduce receptor tyrosine kinase signaling via dimerization and phosphorylation in metazoans. PMID: 24532660
    15. BIK1 mediates inverse functions in plant immunity and development via dynamic association with specific receptor complexes and differential phosphorylation events. PMID: 23818580
    16. BIK1 interacts with PEPR1 and PEPR2 to amplify ethylene-induced immune responses PMID: 23431184
    17. The critical role of BIK1 in plant immune responses hinges upon phosphorylation, its function in ethylene signaling, and complex interactions with other immune response regulators. PMID: 21862710
    18. Xanthomonas campestris pathovar campestris type III effector AvrAC enhances virulence and inhibits plant immunity by specifically targeting Arabidopsis BIK1 and RIPK, two receptor-like cytoplasmic kinases known to mediate immune signalling. PMID: 22504181
    19. Results identify the receptor-like cytoplasmic kinase BIK1 that is rapidly phosphorylated upon flagellin perception, depending on both FLS2 and BAK1. PMID: 20018686
    20. BOTRYTIS-INDUCED KINASE1 (BIK1) modulates the signaling of cellular factors required for defense responses to pathogen infection and normal root hair growth, linking defense response regulation with that of growth and development.[BIK1] PMID: 16339855

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: ath:AT2G39660

    STRING: 3702.AT2G39660.1

    UniGene: At.21254