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  4. Recombinant Arabidopsis thaliana Serine/threonine-protein kinase PBS1 (PBS1)

Recombinant Arabidopsis thaliana Serine/threonine-protein kinase PBS1 (PBS1)

  • 中文名称：
    拟南芥PBS1重组蛋白
  • 货号：
    CSB-YP880708DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PBS1重组蛋白
  • 货号：
    CSB-EP880708DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PBS1重组蛋白
  • 货号：
    CSB-EP880708DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PBS1重组蛋白
  • 货号：
    CSB-BP880708DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PBS1重组蛋白
  • 货号：
    CSB-MP880708DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PBS1
  • Uniprot No.：
  • 别名：
    PBS1; At5g13160; T19L5.120; Serine/threonine-protein kinase PBS1; EC 2.7.11.1; AvrPphB susceptible protein 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-456
  • 氨基酸序列
    MGCFSCFDSS DDEKLNPVDE SNHGQKKQSQ PTVSNNISGL PSGGEKLSSK TNGGSKRELL LPRDGLGQIA AHTFAFRELA AATMNFHPDT FLGEGGFGRV YKGRLDSTGQ VVAVKQLDRN GLQGNREFLV EVLMLSLLHH PNLVNLIGYC ADGDQRLLVY EFMPLGSLED HLHDLPPDKE ALDWNMRMKI AAGAAKGLEF LHDKANPPVI YRDFKSSNIL LDEGFHPKLS DFGLAKLGPT GDKSHVSTRV MGTYGYCAPE YAMTGQLTVK SDVYSFGVVF LELITGRKAI DSEMPHGEQN LVAWARPLFN DRRKFIKLAD PRLKGRFPTR ALYQALAVAS MCIQEQAATR PLIADVVTAL SYLANQAYDP SKDDSRRNRD ERGARLITRN DDGGGSGSKF DLEGSEKEDS PRETARILNR DINRERAVAE AKMWGESLRE KRRQSEQGTS ESNSTG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein kinase required for plant defense mechanism mediated by the disease resistance (R) protein RPS5. In case of infection by Pseudomonas syringae, AvrPphB triggers RPS5-mediated defense mechanism via the cleavage of PBS1. Both kinase activity and cleavage by avrPphB are independently required to trigger the RPS5-mediated resistance. Contributes to PAMP-triggered immunity (PTI) signaling and defense responses downstream of FLS2.
  • 基因功能参考文献：
    1. PBS1 is targeted to the plasma membrane via S-acylation at its N terminus and that plasma membrane localization is required for RPS5 activation. PMID: 24225654
    2. Activation of RPS5 did not require PBS1 kinase function, and thus RPS5 appears to sense a subtle conformational change in PBS1, rather than cleavage. PMID: 22372664
    3. The pathogenic Pseudomonas syringae effector AvrPphB cleaves the Arabidopsis receptor-like cytoplasmic kinase PBS1 to trigger cytoplasmic immune receptor RPS5-specified effector-triggered immunity (ETI). PMID: 20413097
  • 亚细胞定位：
    Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 数据库链接：

    KEGG: ath:AT5G13160

    STRING: 3702.AT5G13160.1

    UniGene: At.23518