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  4. Recombinant Arabidopsis thaliana Transcription factor AS1 (AS1)

Recombinant Arabidopsis thaliana Transcription factor AS1 (AS1)

  • 中文名称：
    Recombinant Arabidopsis thaliana Transcription factor AS1(AS1),Yeast
  • 货号：
    CSB-YP528050DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Transcription factor AS1(AS1),Yeast
  • 货号：
    CSB-EP528050DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Transcription factor AS1(AS1),Yeast
  • 货号：
    CSB-EP528050DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Transcription factor AS1(AS1),Yeast
  • 货号：
    CSB-BP528050DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Transcription factor AS1(AS1),Yeast
  • 货号：
    CSB-MP528050DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    AS1
  • Uniprot No.：
  • 别名：
    AS1; MYB91; PHAN; At2g37630; F13M22.13Transcription factor AS1; Myb-related protein 91; AtMYB91; Protein ASYMMETRIC LEAVES 1; Protein PHANTASTICA; AtPHAN
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-367
  • 氨基酸序列
    MKERQRWSGE EDALLRAYVR QFGPREWHLV SERMNKPLNR DAKSCLERWK NYLKPGIKKG SLTEEEQRLV IRLQEKHGNK WKKIAAEVPG RTAKRLGKWW EVFKEKQQRE EKESNKRVEP IDESKYDRIL ESFAEKLVKE RSNVVPAAAA AATVVMANSN GGFLHSEQQV QPPNPVIPPW LATSNNGNNV VARPPSVTLT LSPSTVAAAA PQPPIPWLQQ QQPERAENGP GGLVLGSMMP SCSGSSESVF LSELVECCRE LEEGHRAWAD HKKEAAWRLR RLELQLESEK TCRQREKMEE IEAKMKALRE EQKNAMEKIE GEYREQLVGL RRDAEAKDQK LADQWTSRHI RLTKFLEQQM GCRLDRP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor required for normal cell differentiation. Positively regulates LATERAL ORGAN BOUNDARIES (LOB) within the shoot apex, and the class III HD-ZIP genes REV, PHB, and PHV. Interacts directly with ASYMMETRIC LEAVES 2 (LBD6/AS2) to repress the knox homeobox genes BP/KNAT1, KNAT2, and KNAT6 and the abaxial determinants ARF3/ETT, KAN2 and YAB5. May act in parallel with the RDR6-SGS3-AGO7 pathway, an endogenous RNA silencing pathway, to regulate the leaf morphogenesis. Binds directly to KNAT1, KNAT2, and KNATM chromatin, regulating leaf development. LBD6 is required for this binding. Positive regulator of flowering that binds to the promoter of FT. Regulates FT expression by forming a functional complex with CO. Involved in leaf polarity establishment by functioning cooperatively with NUCL1 to repress abaxial genes ARF3, ARF4, KAN1, KAN2, YAB1 and YAB5, and the knox homeobox genes KNAT1, KNAT2, KNAT6, and STM to promote adaxial development in leaf primordia at shoot apical meristems at high temperatures.
  • 基因功能参考文献：
    1. that complexes of the transcription factors ASYMMETRIC LEAVES 1 (AS1) and AS2 could help to establish the H3K27me3 modification at the chromatin regions of Class-I KNOTTED1-like homeobox (KNOX) genes BREVIPEDICELLUS and KNAT2 via direct interactions with LHP1. PMID: 27273574
    2. critical for the proper placement of the floral organ abscission zones, and influences the timing of organ shedding PMID: 25038814
    3. CONSTANS (CO) forms a functional complex with ASYMMETRIC LEAVES 1 (AS1) to regulate FLOWERING LOCUS T (FT) expression and that AS1 plays different roles in two regulatory pathways, both of which concomitantly regulate the precise timing of flowering. PMID: 21950734
    4. Negative transcriptional, post-transcriptional and epigenetic regulation of ARF3 by AS1-AS2 is important for stabilizing early leaf partitioning into abaxial and adaxial domains. PMID: 23571218
    5. Taken together, these data indicate that HDA6 is a part of the AS1 repressor complex to regulate the KNOX expression in leaf development. PMID: 23271976
    6. AS1 transcriptional control of meristem cell-specific genes is inhibited by Calmodulin. PMID: 22554014
    7. The JLO and AS2 proteins interact molecularly and form multimeric complexes with AS1 to suppress KNOX expression. AS2 together with JLO regulate auxin transport in seedling roots. PMID: 22822207
    8. In combination with mutations in the MYB domain transcription factor gene ASYMMETRIC LEAVES1 (AS1), partial loss of EMBRYO DEFECTIVE DEVELOPMENT1 (EDD1) function results in leaves with reduced adaxial fate. PMID: 22791832
    9. Data show that TCP3 directly activates the expression of genes for miR164, ASYMMETRIC LEAVES1 (AS1), INDOLE-3-ACETIC ACID3/SHORT HYPOCOTYL2 (IAA3/SHY2), and SMALL AUXIN UP RNA (SAUR) proteins. PMID: 21119060
    10. Results revealed that the reduction in leaf size and late flowering were caused by the repression, by KNOX genes, of a gibberellin (GA) pathway in as1 and as2 plants. PMID: 19891706
    11. Our data suggest that RS2/AS1 and HIRA mediate the epigenetic silencing of knox genes, possibly by modulating chromatin structure. PMID: 16243907
    12. RDR6, SGS3 and AGO7 act in the same pathway, which genetically interacts with the AS1-AS2 pathway for leaf development. PMID: 16699177
    13. AS1 patterns the Arabidopsis gynoecium by repressing BP. PMID: 17592013
    14. function of AS1 in responses to phytopathogens is independent of its AS2-associated role in development PMID: 18003921
    15. A previously unrecognized fundamental regulation by which AS1, AS2, and JAG act to define sepal and petal from their boundaries is reported. PMID: 18156293
    16. AS1 and AS2 form a repressor complex that binds directly to the regulatory motifs CWGTTD and KMKTTGAHW present at two sites in the promoters of the KNOX genes BREVIPEDICELLUS (BP) and KNAT2. PMID: 18203921
    17. results of studying new alleles of AS1 and AS2 support their role in control of class I KNOX genes and auxin transport. PMID: 18409376
    18. AS1, AS2 and the AS1-AS2 protein complex may have distinct functions, which are all required for normal plant development. PMID: 18713400

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in roots, stems, leaves, flowers, siliques and in lateral organ promordia. Found in the inner domain between the adaxial and abaxial domains of leaves. Expressed in the phloem tissues of leaves, cotyledons, hypocotyls, and roots.
  • 数据库链接：

    KEGG: ath:AT2G37630

    STRING: 3702.AT2G37630.1

    UniGene: At.11577