Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Arabidopsis thaliana Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT)

Recombinant Arabidopsis thaliana Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR (FIT)

  • 中文名称：
    拟南芥FIT重组蛋白
  • 货号：
    CSB-YP611716DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥FIT重组蛋白
  • 货号：
    CSB-EP611716DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥FIT重组蛋白
  • 货号：
    CSB-EP611716DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥FIT重组蛋白
  • 货号：
    CSB-BP611716DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥FIT重组蛋白
  • 货号：
    CSB-MP611716DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    FIT
  • Uniprot No.：
  • 别名：
    FIT; BHLH29; EN43; FIT1; FRU; At2g28160; F24D13.5; Transcription factor FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR; Basic helix-loop-helix protein 29; AtbHLH29; bHLH 29; FER-LIKE REGULATOR OF IRON UPTAKE; Transcription factor EN 43; Transcription factor Fe-DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1; bHLH transcription factor bHLH029
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-318
  • 氨基酸序列
    MEGRVNALSN INDLELHNFL VDPNFDQFIN LIRGDHQTID ENPVLDFDLG PLQNSPCFID ENQFIPTPVD DLFDELPDLD SNVAESFRSF DGDSVRAGGE EDEEDYNDGD DSSATTTNND GTRKTKTDRS RTLISERRRR GRMKDKLYAL RSLVPNITKM DKASIVGDAV LYVQELQSQA KKLKSDIAGL EASLNSTGGY QEHAPDAQKT QPFRGINPPA SKKIIQMDVI QVEEKGFYVR LVCNKGEGVA PSLYKSLESL TSFQVQNSNL SSPSPDTYLL TYTLDGTCFE QSLNLPNLKL WITGSLLNQG FEFIKSFT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor. Essential protein involved in iron uptake responses. Regulates FRO2 at the level of mRNA accumulation and IRT1 at the level of protein accumulation. Confers enhanced iron mobilization responses at low iron supply.
  • 基因功能参考文献：
    1. 34 genes were robustly FIT-regulated in root and seedling samples and included 13 novel FIT-dependent genes. PMID: 27716045
    2. FIT interacts with ZAT12 linking iron deficiency and oxidative stress responses. PMID: 26556796
    3. FER-like iron deficiency-induced transcription factor functions as a direct or indirect modulator of protein levels of a subset of gene products under iron deficiency. PMID: 25951126
    4. Nitric oxide as a signal that promoted not only the activation of FIT but also its protein stability, independent of the transcriptional control by ethylene and Nitric oxide. PMID: 21972265
    5. bHLH100 and bHLH101 are key regulators of iron-deficiency responses independent of the master regulator FIT PMID: 22984573
    6. FIT is post-translationally regulated by iron deficiency, leading to a decrease in FIT protein stability mediated by proteasome-dependent degradation. PMID: 21426424
    7. We propose a new model for iron uptake in Arabidopsis where FRO2 and IRT1 are differentially regulated by FIT1 which encodes a putative transcription factor that regulates iron uptake responses in Arabidopsis thaliana [FIT1] PMID: 15539473
    8. AtbHLH29 encods a bHLH protein, which is a functional ortholog of the tomato FER gene which are proposed as transcriptional factors functioning in iron deficiency responses and iron uptake. [AtbHLH29] PMID: 16117851
    9. A proposed common name for At2g28160 in Arabidopsis, namely FIT=FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR, is discussed. PMID: 17466530
    10. ferric-chelate reductase FRO2 and ferrous-transporter IRT1 are the targets of the three transcription factors and the transcription of FRO2 and IRT1 is directly regulated by a complex of FIT/AtbHLH38 or FIT/AtbHLH39. PMID: 18268542

    显示更多

    收起更多

  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in roots and inflorescence, and to a lower extent, in leaves and stems. In roots, confined to the outer cell layers, specifically in the differentiation zone. Also detected in the endodermis and inner tissues of the central cylinder.
  • 数据库链接：

    KEGG: ath:AT2G28160

    STRING: 3702.AT2G28160.1

    UniGene: At.43510