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  4. Recombinant Arabidopsis thaliana Transcription factor SPEECHLESS (SPCH)

Recombinant Arabidopsis thaliana Transcription factor SPEECHLESS (SPCH)

  • 货号：
    CSB-YP765097DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP765097DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP765097DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP765097DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP765097DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPCH
  • Uniprot No.：
  • 别名：
    SPCH; BHLH98; EN19; At5g53210; MFH8.15; Transcription factor SPEECHLESS; Basic helix-loop-helix protein 98; AtbHLH98; bHLH 98; Transcription factor EN 19; bHLH transcription factor bHLH098
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-364
  • 氨基酸序列
    MQEIIPDFLE ECEFVDTSLA GDDLFAILES LEGAGEISPT AASTPKDGTT SSKELVKDQD YENSSPKRKK QRLETRKEED EEEEDGDGEA EEDNKQDGQQ KMSHVTVERN RRKQMNEHLT VLRSLMPCFY VKRGDQASII GGVVEYISEL QQVLQSLEAK KQRKTYAEVL SPRVVPSPRP SPPVLSPRKP PLSPRINHHQ IHHHLLLPPI SPRTPQPTSP YRAIPPQLPL IPQPPLRSYS SLASCSSLGD PPPYSPASSS SSPSVSSNHE SSVINELVAN SKSALADVEV KFSGANVLLK TVSHKIPGQV MKIIAALEDL ALEILQVNIN TVDETMLNSF TIKIGIECQL SAEELAQQIQ QTFC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor acting as an integration node for stomata and brassinosteroid (BR) signaling pathways to control stomatal initiation and development. Activates transcription when in the presence of SCRM/ICE1. Functions as a dimer with SCRM or SCRM2 during stomatal initiation. Required for the initiation, the spacing and the formation of stomata, by promoting the first asymmetric cell divisions. Together with FMA and MUTE, modulates the stomata formation. Involved in the regulation of growth reduction under osmotic stress (e.g. mannitol), associated with a quick decrease of meristemoid mother cells (MMCs) number lower stomatal index and density.
  • 基因功能参考文献：
    1. SPCH role in stomatal development PMID: 28507175
    2. Phosphorylation at Serine 186 is positively required for SPCH function in regulating stomatal development. PMID: 25680231
    3. Arabidopsis reduces growth under stress by integrating the osmotic stress signal into the MAPK-SPEECHLESS core developmental pathway. PMID: 25381317
    4. demonstrate that through phosphorylation inputs from both MAPKs and BIN2, SPCH serves as an integration node for stomata and BR signalling pathways to control stomatal development in Arabidopsis PMID: 22466366
    5. findings show that limited stem cell behavior of stomatal precursors depends on maintenance of the SPEECHLESS transcription factor in single daughter cells PMID: 21903812
    6. study found that a unique domain in basic helix-loop-helix (bHLH) stomatal initiating factor, SPEECHLESS, renders it a mitogen-activated protein kinase phosphorylation target in vitro and modulates its function in vivo PMID: 19008449

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in developing leaf epidermis. Reduced accumulation in the stomatal lineage ground cells (SLGCs) where BASL is polarized in the cell cortex. Observed in small cells of non-protruding hypocotyl cell files and of developing cotyledon epidermis. Res
  • 数据库链接：

    KEGG: ath:AT5G53210

    STRING: 3702.AT5G53210.1

    UniGene: At.50528