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  4. Recombinant Archaeoglobus fulgidus DNA-directed RNA polymerase subunit M (rpoM)

Recombinant Archaeoglobus fulgidus DNA-directed RNA polymerase subunit M (rpoM)

  • 中文名称：
    富尔古布氏菌tfs重组蛋白
  • 货号：
    CSB-YP516116DOC
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    富尔古布氏菌tfs重组蛋白
  • 货号：
    CSB-EP516116DOC
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    富尔古布氏菌tfs重组蛋白
  • 货号：
    CSB-EP516116DOC-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    富尔古布氏菌tfs重组蛋白
  • 货号：
    CSB-BP516116DOC
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    富尔古布氏菌tfs重组蛋白
  • 货号：
    CSB-MP516116DOC
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tfs
  • Uniprot No.：
  • 别名：
    tfs; AF_1235; Transcription factor S; Transcription elongation factor IIS/RNA polymerase subunit homolog; TFIIS/RPSU homolog
  • 种属：
    Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-103
  • 氨基酸序列
    MEFCPKCKSL MIYQGDKLVC RKCGYEKEAD DSEELVIKVE RNKEDVPVIE GENLKTLPTT KAICPACGHN EAFWWLRQLR AADESEVRFF RCTKCGKTWR EYD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Induces RNA cleavage activity in the RNA polymerase. In its presence, the cleavage activity of the RNA polymerase truncates the RNA back to position +15 in a stepwise manner by releasing mainly dinucleotides from the 3'-end of the nascent RNA. The truncated RNAs are able to continue elongation. Involved in transcriptional proofreading and fidelity. Misincorporation of nucleotides during elongation of transcription leads to arrested elongation complexes which are rescued by TFS-promoted removal of a dinucleotide from the 3'-end. TFS is able to induce a cleavage resynthesis cycle in stalled elongation complexes (resulting from the next missing nucleotide or a reduced incorporation rate of a wrong nucleotide) preventing misincorporation and enabling proofreading in a post-incorporation manner. Pausing of elongation complexes is the main determinant of TFS-induced RNA cleavage.
  • 蛋白家族：
    Archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family
  • 数据库链接：

    KEGG: afu:AF_1235

    STRING: 224325.AF1235