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  4. Recombinant Avian infectious bronchitis virus Nucleoprotein (N)

Recombinant Avian infectious bronchitis virus Nucleoprotein (N)

  • 货号：
    CSB-YP304834ARN
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP304834ARN
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP304834ARN-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP304834ARN
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP304834ARN
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    N; 6; Nucleoprotein; Nucleocapsid protein; NC; Protein N
  • 种属：
    Avian infectious bronchitis virus (strain Beaudette) (IBV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-409
  • 氨基酸序列
    MASGKAAGKT DAPAPVIKLG GPKPPKVGSS GNASWFQAIK AKKLNTPPPK FEGSGVPDNE NIKPSQQHGY WRRQARFKPG KGGRKPVPDA WYFYYTGTGP AADLNWGDTQ DGIVWVAAKG ADTKSRSNQG TRDPDKFDQY PLRFSDGGPD GNFRWDFIPL NRGRSGRSTA ASSAAASRAP SREGSRGRRS DSGDDLIARA AKIIQDQQKK GSRITKAKAD EMAHRRYCKR TIPPNYRVDQ VFGPRTKGKE GNFGDDKMNE EGIKDGRVTA MLNLVPSSHA CLFGSRVTPK LQLDGLHLRF EFTTVVPCDD PQFDNYVKIC DQCVDGVGTR PKDDEPKPKS RSSSRPATRG NSPAPRQQRP KKEKKLKKQD DEADKALTSD EERNNAQLEF YDEPKVINWG DAALGENEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
  • 基因功能参考文献：
    1. The potential interactome of the N protein was mapped using stable isotope labeling with amino acids in cell culture (SILAC) coupled to a green fluorescent protein-nanotrap pulldown methodology and liquid chromatography-tandem mass spectrometry. PMID: 23637410
    2. s show that phosphorylation at Thr378 and Ser379 sites is dependent on the ataxia-telangiectasia mutated (ATM) and Rad3-related (ATR), a kinase activated during infectious bronchitis virus replication. PMID: 23849791
    3. determination of sequences of N protein genes of IBV isolates in Korea & comparison with those of non-Korean IBV strains PMID: 16025240
    4. The crystal structure of the IBV-N29-160 fragment at 1.85 A resolution was reported. PMID: 16338414
    5. Identification of residues critical for RNA binding on the nucleocapsid surface, and biochemical and genetic evidence that directly links the RNA binding capacity of the coronavirus N protein to the viral infectivity in cultured cells. PMID: 16971454
    6. analysis of infectious bronchitis virus nucleoprotein PMID: 17037519
    7. analysis of coronavirus nucleoprotein effect on cell nucleolus by confocal microscopy PMID: 17037551
    8. The amino-terminal region facilitates long-range non-specific interactions between N protein and viral RNA, thus bringing the RNA into close proximity to N protein allowing specific contacts to form via a 'lure' and 'lock' mechanism. PMID: 17052713

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  • 亚细胞定位：
    Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus.
  • 蛋白家族：
    Gammacoronavirus nucleocapsid protein family
  • 数据库链接：

    KEGG: vg:1489745