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  4. Recombinant Bacillus phage phi29 Encapsidation protein (16)

Recombinant Bacillus phage phi29 Encapsidation protein (16)

  • 货号：
    CSB-YP319244BBC
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP319244BBC
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP319244BBC-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP319244BBC
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP319244BBC
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    16
  • Uniprot No.：
  • 别名：
    16; DNA packaging protein; ATPase gp16; EC 3.6.4.-; Gene product 16; gp16; Protein p16
  • 种属：
    Bacillus phage phi29 (Bacteriophage phi-29)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-332
  • 氨基酸序列
    MDKSLFYNPQ KMLSYDRILN FVIGARGIGK SYAMKVYPIN RFIKYGEQFI YVRRYKPELA KVSNYFNDVA QEFPDHELVV KGRRFYIDGK LAGWAIPLSV WQSEKSNAYP NVSTIVFDEF IREKDNSNYI PNEVSALLNL MDTVFRNRER VRCICLSNAV SVVNPYFLFF NLVPDVNKRF NVYDDALIEI PDSLDFSSER RKTRFGRLID GTEYGEMSLD NQFIGDSQVF IEKRSKDSKF VFSIVYNGFT LGVWVDVNQG LMYIDTAHDP STKNVYTLTT DDLNENMMLI TNYKNNYHLR KLASAFMNGY LRFDNQVIRN IAYELFRKMR IQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    ATPase required for the genome encapsidation reaction. Part of the active packaging motor via the binding to the packaging RNA (pRNA), itself fixed to the head-tail connector at the unique portal vertex of the prohead. Binds and supercoils the DNA-gp3 to produce an initiation complex for DNA packaging. Provides the energy to actively pump the viral DNA into the prohead. Approximately one molecule of ATP is used in the packaging of 2 bp of viral DNA. After packaging, the ATPase and the pRNA are released from the prohead.
  • 基因功能参考文献：
    1. Results report that the sequential action of the ATPase ring in the DNA packaging motor of bacteriophage phi29 is regulated by an arginine finger that extends from one ATPase subunit to the adjacent unit to promote noncovalent dimer formation. PMID: 27457616
    2. s confirmed the stoichiometry of phi29 motor ATPase to be a hexamer and provide data suggesting that the phi29 motor ATPase is a member of the classical hexameric AAA+ superfamily. PMID: 23706809
    3. s report a revolution rather than rotation mechanism for the bacteriophage phi29 DNA packaging motor. PMID: 23763768
    4. Kinetic analysis of phi29 DNA packaging motor provides evidence for coordinated intersubunit ATPase activity of gp16. PMID: 22795974
    5. The binding of gp16 to the prohead/pRNA to constitute the complete and active packaging motor was confirmed by cryo-electron microscopy three-dimensional reconstruction of the prohead/pRNA/gp16 complex PMID: 18674782
    6. direct observation of the intersubunit coordination and step size of a ring ATPase, the double-stranded-DNA packaging motor in the bacteriophage phi29 PMID: 19129763
    7. The full mechanochemical cycle of the motor, in which the ATPase is a pentameric-ring of gene product 16, involves two phases-an ATP-loading dwell followed by a translocation burst of four 2.5-base-pair steps triggered by hydrolysis product release. PMID: 19794496

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  • 蛋白家族：
    Phi29likevirus gp16 family
  • 数据库链接：

    KEGG: vg:6446516