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  4. Recombinant Bacillus subtilis HTH-type transcriptional regulatory protein gabR (gabR)

Recombinant Bacillus subtilis HTH-type transcriptional regulatory protein gabR (gabR)

  • 货号：
    CSB-YP309788BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP309788BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP309788BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP309788BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP309788BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    gabR
  • Uniprot No.：
  • 别名：
    gabR; ycnF; BSU03890HTH-type transcriptional regulatory protein GabR
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-479
  • 氨基酸序列
    MDITITLDRS EQADYIYQQI YQKLKKEILS RNLLPHSKVP SKRELAENLK VSVNSVNSAY QQLLAEGYLY AIERKGFFVE ELDMFSAEEH PPFALPDDLK EIHIDQSDWI SFSHMSSDTD HFPIKSWFRC EQKAASRSYR TLGDMSHPQG IYEVRAAITR LISLTRGVKC RPEQMIIGAG TQVLMQLLTE LLPKEAVYAM EEPGYRRMYQ LLKNAGKQVK TIMLDEKGMS IAEITRQQPD VLVTTPSHQF PSGTIMPVSR RIQLLNWAAE EPRRYIIEDD YDSEFTYDVD SIPALQSLDR FQNVIYMGTF SKSLLPGLRI SYMVLPPELL RAYKQRGYDL QTCSSLTQLT LQEFIESGEY QKHIKKMKQH YKEKRERLIT ALEAEFSGEV TVKGANAGLH FVTEFDTRRT EQDILSHAAG LQLEIFGMSR FNLKENKRQT GRPALIIGFA RLKEEDIQEG VQRLFKAVYG HKKIPVTGD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates the transcription of the gabTD operon. Is also a repressor of its own expression, both in the presence and absence of GABA. Binds specifically to the DNA region overlapping the -35 region of the gabT promoter and the -10 and +1 regions of the gabR promoter. Principally regulates the utilization of gamma-aminobutyrate.
  • 基因功能参考文献：
    1. One subunit is a closed-form containing the PLP-GABA adduct, and the other subunit is a PLP-bound open-form. Our structural studies provide a detailed mechanism indicating that the open-to-closed transition by the binding of GABA induces the conformational rearrangement of BsGabR-CTD, which may trigger the activation of transcription. PMID: 28412355
    2. Data show that DNA undergoes a conformational change to bend around transcription factor GabR during binding. PMID: 26681693
    3. These results suggested that each domain of GabR could be an independent folding unit. The C-terminal domain provides the N-terminal domain with DNA-binding ability via dimerization. PMID: 25911692
    4. study examined the mechanism underlying GabR-mediated gabTD transcription, focusing on the function of the aminotransferase domain. PMID: 25388514
  • 蛋白家族：
    Class-I pyridoxal-phosphate-dependent aminotransferase family
  • 数据库链接：

    KEGG: bsu:BSU03890

    STRING: 224308.Bsubs1_010100002193