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  4. Recombinant Bacillus subtilis Peptidoglycan endopeptidase LytF (lytF)

Recombinant Bacillus subtilis Peptidoglycan endopeptidase LytF (lytF)

  • 中文名称：
    枯草芽孢杆菌lytF重组蛋白
  • 货号：
    CSB-YP517446BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌lytF重组蛋白
  • 货号：
    CSB-EP517446BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌lytF重组蛋白
  • 货号：
    CSB-EP517446BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌lytF重组蛋白
  • 货号：
    CSB-BP517446BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌lytF重组蛋白
  • 货号：
    CSB-MP517446BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    lytF
  • Uniprot No.：
  • 别名：
    lytF; cwlE; yhdD; BSU09370Peptidoglycan endopeptidase LytF; EC 3.4.-.-; Autolysin LytF; Cell wall-associated polypeptide CWBP49'; Gamma-D-glutamate-meso-diaminopimelate muropeptidase LytF; Peptidoglycan hydrolase LytF; Vegetative cell wall hydrolase LytF
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-488
  • 氨基酸序列
    ATIK VKSGDSLWKL AQTYNTSVAA LTSANHLSTT VLSIGQTLTI PGSKSSTSSS TSSSTTKKSG SSVYTVKSGD SLWLIANEFK MTVQELKKLN GLSSDLIRAG QKLKVSGTVS SSSSSSKKSN SNKSSSSSSK SSSNKSSSSS SSTGTYKVQL GDSLWKIANK VNMSIAELKV LNNLKSDTIY VNQVLKTKSS GSDTSSKDNS SKSNQTSATT KYTVKSGDSL WKIANNYNLT VQQIRNINNL KSDVLYVGQV LKLTGKASSG SSSSSSSSSN ASSGTTTTYT VKSGDSLWVI AQKFNVTAQQ IREKNNLKTD VLQVGQKLVI SGKASSSSSS GSSNTTSSTS AKINTMISAA KAQLGVPYRW GGTTPSGFDC SGFIYYVLNK VTSVSRLTAA GYWNTMKSVS QPAVGDFVFF STYKAGPSHV GIYLGNGEFI NANDSGVVIS NMNNSYWKQR YLGAKRYF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cell wall hydrolase that cleaves gamma-D-glutamate-meso-diaminopimelate bonds in peptidoglycan. LytF is necessary and sufficient for vegetative daughter cell separation, and also seems to play a role in cell autolysis.
  • 基因功能参考文献：
    1. beta4-3(10) loop of IseA gets stuck deep in the cleft of LytF, and the active site is occluded. PMID: 23091053
    2. These results strongly suggest that wall teichoic acids inhibit the sidewall localization of LytF. PMID: 18761696
  • 亚细胞定位：
    Secreted, cell wall. Note=LytF is localized at cell separation sites and cell poles of rod-shaped cells during vegetative growth.
  • 蛋白家族：
    Peptidase C40 family
  • 数据库链接：

    KEGG: bsu:BSU09370

    STRING: 224308.Bsubs1_010100005173