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  4. Recombinant Bacillus subtilis Peroxide operon regulator (perR)

Recombinant Bacillus subtilis Peroxide operon regulator (perR)

  • 货号：
    CSB-YP304084BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP304084BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP304084BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP304084BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP304084BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    perR
  • Uniprot No.：
  • 别名：
    perR; ygaG; BSU08730; Peroxide operon regulator
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-145
  • 氨基酸序列
    MAAHELKEAL ETLKETGVRI TPQRHAILEY LVNSMAHPTA DDIYKALEGK FPNMSVATVY NNLRVFRESG LVKELTYGDA SSRFDFVTSD HYHAICENCG KIVDFHYPGL DEVEQLAAHV TGFKVSHHRL EIYGVCQECS KKENH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydrogen and organic peroxide sensor. Represses the expression of a regulon of peroxide-inducible genes such as katA, ahpC, ahpF, the heme biosynthesis operon (hemAXCDBL), fur, perR, zosA and mrgA.
  • 基因功能参考文献：
    1. eme accumulation results from dysregulation of the regulon controlled by PerR, a metal-dependent repressor of peroxide stress genes. When metallated with Fe(II) or Mn(II), PerR represses both heme biosynthesis (hemAXCDBL operon) and the abundant heme protein catalase (katA). PMID: 27935957
    2. this study identified a LonA-recognition site critical for oxidation-controlled PerR turnover. PMID: 26677871
    3. A transcriptional srfA-lacZ fusion lacking both PerR boxes showed diminished and PerR-independent expression, indicating that the PerR boxes we identified function as positive cis elements for srfA transcription PMID: 16166527
    4. A model is proposed in which high levels of H2O2 release sufficient ferrous iron (Fe2+) into the cytosol to effect a transition of PerR from the PerR:Zn.Mn form to the peroxide sensititve PerR:Zn.Fe form. PMID: 16766519
    5. A specific histidine residue makes the N- and C-terminal domains tadopt a caliper-like conformation suited to bind DNA. PMID: 19508285

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Fur family
  • 数据库链接：

    KEGG: bsu:BSU08730

    STRING: 224308.Bsubs1_010100004833