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  4. Recombinant Bacillus subtilis Putative ribosome biogenesis GTPase RsgA (rsgA)

Recombinant Bacillus subtilis Putative ribosome biogenesis GTPase RsgA (rsgA)

  • 中文名称：
    枯草芽孢杆菌rsgA重组蛋白
  • 货号：
    CSB-YP516504BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌rsgA重组蛋白
  • 货号：
    CSB-EP516504BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌rsgA重组蛋白
  • 货号：
    CSB-EP516504BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌rsgA重组蛋白
  • 货号：
    CSB-BP516504BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌rsgA重组蛋白
  • 货号：
    CSB-MP516504BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rsgA
  • Uniprot No.：
  • 别名：
    rsgA; cpgA; engC; yloQ; BSU15780; Small ribosomal subunit biogenesis GTPase RsgA; EC 3.6.1.-; Ribosome-associated GTPase CpgA
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-298
  • 氨基酸序列
    MPEGKIIKAL SGFYYVLDES EDSDKVIQCR GRGIFRKNKI TPLVGDYVVY QAENDKEGYL MEIKERTNEL IRPPICNVDQ AVLVFSAVQP SFSTALLDRF LVLVEANDIQ PIICITKMDL IEDQDTEDTI QAYAEDYRNI GYDVYLTSSK DQDSLADIIP HFQDKTTVFA GQSGVGKSSL LNAISPELGL RTNEISEHLG RGKHTTRHVE LIHTSGGLVA DTPGFSSLEF TDIEEEELGY TFPDIREKSS SCKFRGCLHL KEPKCAVKQA VEDGELKQYR YDHYVEFMTE IKDRKPRY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    One of several proteins that assist in the late maturation steps of the functional core of the 30S ribosomal subunit. Helps release RbfA from mature subunits. May play a role in the assembly of ribosomal proteins into the subunit. Circularly permuted GTPase with a low level of activity and slow catalytic turnover, does not act on ATP. GTPase activity is stimulated by the presence of 30S or 70S ribosomes, phosphorylation increases stimulation. Depletion results in increased sensitivity to protein synthesis inhibitors that block the peptide channel or peptidyl transferase center on the ribosome, suggesting this protein functions in conjunction with the ribosome in vivo. Decreasing levels of protein lead to an increase in free 30S and 50S ribosomal subunits and a decrease in assembled 70S ribosomes. Suggested to serve as a specific transcription factor for proteins involved in late stages of peptidoglycan synthesis.
  • 基因功能参考文献：
    1. YLOQ is involved in the translation of a subset of proteins, including some required for shape maintenance PMID: 16485133
    2. CpgA phosphorylated on Thr and Ser is a target for both PrkC and PrpC in vitro. PMID: 19246764
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    TRAFAC class YlqF/YawG GTPase family, RsgA subfamily
  • 数据库链接：

    KEGG: bsu:BSU15780

    STRING: 224308.Bsubs1_010100008711