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  4. Recombinant Bacillus subtilis Sec-independent protein translocase protein TatAd (tatAd), partial

Recombinant Bacillus subtilis Sec-independent protein translocase protein TatAd (tatAd), partial

  • 中文名称：
    枯草芽孢杆菌tatAd重组蛋白
  • 货号：
    CSB-YP519977BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌tatAd重组蛋白
  • 货号：
    CSB-EP519977BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌tatAd重组蛋白
  • 货号：
    CSB-EP519977BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌tatAd重组蛋白
  • 货号：
    CSB-BP519977BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌tatAd重组蛋白
  • 货号：
    CSB-MP519977BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tatAd
  • Uniprot No.：
  • 别名：
    tatAd; yczB; BSU02630; Sec-independent protein translocase protein TatAd
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system. Required for PhoD secretion. The cytosolic fraction of TatAd binds the precursor of PhoD.
  • 基因功能参考文献：
    1. There is an N-terminal TatB-type domain within TatAd, together with a separate domain that appears to be universally conserved among TatA/TatB proteins. PMID: 21479530
    2. the solution structure of the TatA(d) protein from Bacillus subtilis by NMR spectroscopy is reported. PMID: 20726548
    3. This finding implies that the amphiphilic region of TatA is not just a flexible attachment to the transmembrane anchor but might be able to form intra- or even intermolecular salt-bridges, which could play a key role in pore assembly. PMID: 20977272
    4. The topology and orientation of TatAd was determined in an environment mimicking the bacterial membrane, and a wheel-like pattern characteristic for a tilted transmembrane helix, was observed in 15N chemical shift /15N-1H dipolar coupling correlation NMR. PMID: 17980349
    5. The results suggest that the formation of TatAd complexes in B. subtilis is controlled by TatC. PMID: 19395490

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  • 亚细胞定位：
    Cell membrane; Single-pass membrane protein. Cytoplasm, cytosol. Note=At the onset of phosphate starvation, substantial amounts of TatAd are present in the cytosol. Upon ongoing starvation, the amount of TatAd in the cytosol decreases.
  • 蛋白家族：
    TatA/E family
  • 数据库链接：

    KEGG: bsu:BSU02630

    STRING: 224308.Bsubs1_010100001463