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  4. Recombinant Bacillus subtilis Transition state regulatory protein AbrB (abrB)

Recombinant Bacillus subtilis Transition state regulatory protein AbrB (abrB)

  • 货号：
    CSB-YP357507BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP357507BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP357507BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP357507BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP357507BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    abrB
  • Uniprot No.：
  • 别名：
    abrB; cpsX; BSU00370Transition state regulatory protein AbrB
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-96
  • 氨基酸序列
    MFMKSTGIVR KVDELGRVVI PIELRRTLGI AEKDALEIYV DDEKIILKKY KPNMTCQVTG EVSDDNLKLA GGKLVLSKEG AEQIISEIQN QLQNLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ambiactive repressor and activator of the transcription of genes expressed during the transition state between vegetative growth and the onset of stationary phase and sporulation. It controls the expression of genes spovG and tycA. AbrB binds to the tycA promoter region at two A- and T-rich sites, it may be the sole repressor of tycA transcription.
  • 基因功能参考文献：
    1. we show that the sRNA-induced diversity in AbrB levels generates heterogeneity in growth rates during the exponential growth phase PMID: 25790031
    2. C-termini are responsible for the cooperative character observed for AbrB interaction with some DNA targets like tycA and phyC PMID: 24832089
    3. this study solved the NMR structures of both domains independently and utilized gas-phase cleavable chemical crosslinking and LC/MS(n) analysis to correctly position the domains within the full tetrameric AbrB protein structure. PMID: 23313475
    4. A report on the in vivo distribution over the entire B. subtilis genome of AbrB-binding sites during exponential growth phase when AbrB principally functions. PMID: 20817675
    5. we demonstrate that overproduction of the phosphorelay components KinA and Spo0A stimulates cell bundle formation in biofilms, while overproduction of the transition state regulators AbrB and SinR leads to repression of formation of elevated bundles. PMID: 16585769
    6. Abh gene, controling AbrB expression is part of the complex interconnected regulatory system that controls gene expression during the transition from active growth to stationary phase. PMID: 17720793

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  • 数据库链接：

    KEGG: bsu:BSU00370

    STRING: 224308.Bsubs1_010100000185