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  4. Recombinant Bacillus subtilis Uncharacterized protein skfB (skfB)

Recombinant Bacillus subtilis Uncharacterized protein skfB (skfB)

  • 中文名称：
    枯草芽孢杆菌skfB重组蛋白
  • 货号：
    CSB-YP516248BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌skfB重组蛋白
  • 货号：
    CSB-EP516248BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌skfB重组蛋白
  • 货号：
    CSB-EP516248BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌skfB重组蛋白
  • 货号：
    CSB-BP516248BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌skfB重组蛋白
  • 货号：
    CSB-MP516248BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    skfB
  • Uniprot No.：
  • 别名：
    skfB; ybcP; ybcQ; BSU01920; Sporulation killing factor maturation protein SkfB; EC 1.21.98.-
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-410
  • 氨基酸序列
    MSYDRVKDFD LPELAVHLQP HGAVMIDRKS MFYFRLSGRG AQLAFLLSKN KNLHKTARIW EIMKKEEMSA DQLKEELSAH PFTEAWTEGL LDQPLHVSGS LDSYLPISCT LQLTNACNLS CSFCYASSGK PYPEELSSEQ WILVMQKLAA HGVADITLTG GEAKLIKGFK ELVVVASSLF TNVNVFSNGL NWRDEEVELL SHLGNVSVQI SIDGMDNTHD QLRGRKGGFK ESMNTIKKLS EANIPVIVAM TINESNADEV SDVVEQCANA GAFIFRAGKT LSVGRATEGF KALDIDFEEM VQIQLREARH KWGDRLNIID WEHEESSFTT DFCTPGYLAW YIRADGYVTP CQLEDLPLGH ILEDSMADIG SPARLLQLKC EAKNCKCIGK IELSEPDLPF QKEVKAGIQE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the formation of the thioether bond required for production of the sporulation killing factor (SKF) from SkfA. Forms the cysteine-methionine thioether bond found in SKF; the acceptor amino acid can be hydrophobic, aromatic or a small hydrophilic amino acid but not a larger hydrophilic amino acid, i.e. Met=Ala, Phe, Leu, Tyr>Asn, Ser>>Gln, Glu, Lys. The relative position of Cys and Met in the substrate cannot be inverted, in vitro the thioether bond cannot be made in the absence of the SkfA propeptide, suggesting this is the first reaction in SKF maturation. In vitro, in the absence of a second substrate, cleaves S-adenosyl-L-methionine into Met and 5'-dA.
  • 基因功能参考文献：
    1. The radical SAM enzyme, SkfB, utilizes the 5'-deoxyadenosyl radical generated from the reductive cleavage of S-adenosyl-l-methionine to abstract hydrogen atom from the alpha-carbon of the amino acid at position 12 in the substrate, SkfA, to initiate the installation of a thioether cross-link. PMID: 27410522
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Radical SAM superfamily
  • 数据库链接：

    KEGG: bsu:BSU01920

    STRING: 224308.Bsubs1_010100001083