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  4. Recombinant Bovine COUP transcription factor 2 (NR2F2)

Recombinant Bovine COUP transcription factor 2 (NR2F2)

  • 中文名称：
    牛NR2F2重组蛋白
  • 货号：
    CSB-YP874679BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    牛NR2F2重组蛋白
  • 货号：
    CSB-EP874679BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    牛NR2F2重组蛋白
  • 货号：
    CSB-EP874679BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    牛NR2F2重组蛋白
  • 货号：
    CSB-BP874679BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    牛NR2F2重组蛋白
  • 货号：
    CSB-MP874679BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NR2F2
  • Uniprot No.：
  • 别名：
    NR2F2; TFCOUP2COUP transcription factor 2; COUP-TF2; COUP transcription factor II; COUP-TF II; Nuclear receptor subfamily 2 group F member 2
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-414
  • 氨基酸序列
    MAMVVSTWRD PQDEVPGSQG SQASQAPPVP GPPPGAPHTP QTPGQGGPAS TPAQTAAGGQ GGPGGPGSDK QQQQQHIECV VCGDKSSGKH YGQFTCEGCK SFFKRSVRRN LSYTCRANRN CPIDQHHRNQ CQYCRLKKCL KVGMRREAVQ RGRMPPTQPS HGQFALTNGD PLNCHSYLSG YISLLLRAEP YPTSRFGSQC MQPNNIMGIE NICELAARML FSAVEWARNI PFFPDLQITD QVALLRLTWS ELFVLNAAQC SMPLHVAPLL AAAGLHASPM SADRVVAFMD HIRIFQEQVE KLKALHVDSA EYSCLKAIVL FTSDACGLSD VAHVESLQEK SQCALEEYVR SQYPNQPTRF GKLLLRLPSL RTVSSSVIEQ LFFVRLVGKT PIETLIRDML LSGSSFNWPY MAIQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-EP874679BO, could you please advise on the pricing, the lead time, the sequencing, and the expected tag information for all 4 expression hosts?
Also, I am wondering about purity and “solubility” data for this protein. Does CUSA ever characterize any solubility data for the proteins other than just making sure that it is soluble?

A:
Thanks for your inquiry.
Recombinant Bovine COUP transcription factor 2(NR2F2)
CSB-YP874679BO >> Yeast
CSB-EP874679BO >> E.coli
CSB-BP874679BO >> Baculovirus
CSB-MP874679BO >> Mammalian cell
Expression Region:1-414aa; Full length.
Tag information:EP, YP, BP, MP: Tag type will be determined during the manufacturing process.
The expected tag for each expression system is listed as follows:
YP: N-terminal 6xHis-tagged; EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

MAMVVSTWRDPQDEVPGSQGSQASQAPPVPGPPPGAPHTPQTPGQGGPASTPAQTAAGGQGGPGGPGSDKQQQQQHIECVVCGDKSSGKHYGQFTCEGCKSFFKRSVRRNLSYTCRANRNCPIDQHHRNQCQYCRLKKCLKVGMRREAVQRGRMPPTQPSHGQFALTNGDPLNCHSYLSGYISLLLRAEPYPTSRFGSQCMQPNNIMGIENICELAARMLFSAVEWARNIPFFPDLQITDQVALLRLTWSELFVLNAAQCSMPLHVAPLLAAAGLHASPMSADRVVAFMDHIRIFQEQVEKLKALHVDSAEYSCLKAIVLFTSDACGLSDVAHVESLQEKSQCALEEYVRSQYPNQPTRFGKLLLRLPSLRTVSSSVIEQLFFVRLVGKTPIETLIRDMLLSGSSFNWPYMAIQ


Sorry, as we haven't expressed the above proteins for the time being, so we can't provide the purity data.
The minimum purity we can guarantee is 85%, however we will try for high purity.Our purity standard of the QC is 85%, so if it's finally can only reach around 85%, we will also take it as a qualified product.
Sorry, for the time being, we haven't characterized any solubility data for the protein, we just guarantee it is soluble.

靶点详情

  • 功能：
    Ligand-activated transcription factor. Activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid, but not by dexamethasone, cortisol or progesterone (in vitro). Regulation of the apolipoprotein A-I gene transcription. Binds to DNA site A. May be required to establish ovary identity during early gonad development.
  • 基因功能参考文献：
    1. Results show that chicken ovalbumin upstream promoter transcription factor II (COUP-TFII), hepatocyte nuclear factor 4alpha (HNF-4alpha) and HNF-4gamma regulate growth hormone receptor 1A promoter activity by binding to a common DNA element PMID: 15171724
    2. Involvement of Ad4BP/SF-1, DAX-1, and COUP-TFII transcription factor on steroid production and luteinization in ovarian theca cells. PMID: 18409030
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Nuclear hormone receptor family, NR2 subfamily
  • 数据库链接：

    KEGG: bta:281945

    STRING: 9913.ENSBTAP00000023969

    UniGene: Bt.53763