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  4. Recombinant Bovine Collagenase 3 (MMP13)

Recombinant Bovine Collagenase 3 (MMP13)

  • 货号：
    CSB-YP014660BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP014660BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP014660BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP014660BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP014660BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    MMP13; Collagenase 3; EC 3.4.24.-; Matrix metalloproteinase-13; MMP-13
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    104-471
  • 氨基酸序列
    YNVFPRT LKWSKMNLTY RIVNYTPDLT HSEVEKAFRK AFKVWSDVTP LNFTRIHNGT ADIMISFGTK EHGDFYPFDG PSGLLAHAFP PGPNYGGDAH FDDDETWTSS SKGYNLFLVA AHEFGHSLGL DHSKDPGALM FPIYTYTGKS HFMLPDDDVQ GIQSLYGPGD EDPYSKHPKT PDKCDPSLSL DAITSLRGET LIFKDRFFWR LHPQQVEAEL FLTKSFGPEL PNRIDAAYEH PSHDLIFIFR GRKFWALSGY DILEDYPKKI SELGFPKHVK KISAALHFED SGKTLFFSEN QVWSYDDTNH VMDKDYPRLI EEVFPGIGDK VDAVYQKNGY IYFFNGPIQF EYSIWSNRIV RVMTTNSLLW C
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
  • 基因功能参考文献：
    1. p38 phosphorylation and MMP13 expression are regulated by Rho/ROCK activation, and support the potential novel pathway that Rho/ROCK is in the upper part of the mechanical stress-induced matrix degeneration cascade in cartilage. PMID: 22688963
    2. These data identify atypical PKC isozymes as STAT and ERK activators that mediate c-fos and collagenase expression. PMID: 20463008
    3. MMP-13 treatment of fresh articular cartilage results in cleaved fibromodulin fragments PMID: 14660626
    4. increased in bovine preovulatory follicles following the gonadotropin surge but no up-regulation of MMP-1 and MMP-13 (follicular apex vs. base) for the preovulatory collagenolysis required for follicle rupture PMID: 15115716
    5. involvement of p38 MAP kinase in the hyaluronan oligosaccharide induction of MMP-13 PMID: 16648633
    6. MMP-13 and collagenase have roles in chondrocyte hypertrophy induced by type II collagen PMID: 17306969
    7. At high but naturally occurring concentrations the collagen peptide CB12-II induced an increase in the expressions of MMP-13 and cleavage of type II collagen by collagenase in the mid zone and also in the superficial zone. PMID: 18589731

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  • 亚细胞定位：
    Secreted, extracellular space, extracellular matrix. Secreted.
  • 蛋白家族：
    Peptidase M10A family
  • 数据库链接：

    KEGG: bta:281914

    STRING: 9913.ENSBTAP00000020045

    UniGene: Bt.39