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  4. Recombinant Bovine DNA damage-binding protein 2 (DDB2)

Recombinant Bovine DNA damage-binding protein 2 (DDB2)

  • 货号：
    CSB-YP604366BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP604366BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP604366BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP604366BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP604366BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DDB2; DNA damage-binding protein 2; Damage-specific DNA-binding protein 2
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-426
  • 氨基酸序列
    MAPRKRPENQ KTPEVVVRPK SKRNRSPREL EPEAKKLCVK GPGSSRRFDS GLWAGLASLR VPPLCSSIVR ALHQHKLGTA AWPSLQQGLQ QSFLNSLASY RIFQKAAPFD RRATSLAWHP THPSTLAVGS KGGDILLWNF GIKDKPTFIK GIGAGGSITG MKFNPLNTNQ FFTSSMEGTT RLQDFKGNTL RVFASSDTCN VWFCSLDVSV KSRVVVTGDN VGHVILLNMD GRELWNLRMH KKKVTHVALN PCCDWLLATA SVDQTVKIWD LRQVRGKSSF LHSLPHRHPV NAAHFSPDGA QLLTTDQKSE IRVYSACQWD CPPSLIPHPH RHFQHLTPIK ASWHPRYNLI VVGRYPDPNF KSCSPHELRT IDVFDGSSGK IMYQLYDPES SGIMSLNEFN PMGDTLASVM GYHILVWSPE DAGTQK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein, which is both involved in DNA repair and protein ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box) complexes, respectively. Core component of the UV-DDB complex (UV-damaged DNA-binding protein complex), a complex that recognizes UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also functions as the substrate recognition module for the DCX (DDB2-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB2-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. The DDB2-CUL4-ROC1 complex also ubiquitinates KAT7/HBO1 in response to DNA damage, leading to its degradation: recognizes KAT7/HBO1 following phosphorylation by ATR.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WD repeat DDB2/WDR76 family
  • 数据库链接：

    KEGG: bta:519357

    STRING: 9913.ENSBTAP00000027965

    UniGene: Bt.33161