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Recombinant Bovine S-arrestin (SAG)

  • 中文名称：
    牛SAG重组蛋白
  • 货号：
    CSB-YP020669BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    牛SAG重组蛋白
  • 货号：
    CSB-EP020669BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    牛SAG重组蛋白
  • 货号：
    CSB-EP020669BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    牛SAG重组蛋白
  • 货号：
    CSB-BP020669BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    牛SAG重组蛋白
  • 货号：
    CSB-MP020669BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SAG
  • Uniprot No.：
  • 别名：
    SAG; S-arrestin; 48 kDa protein; Retinal S-antigen; S-AG; Rod photoreceptor arrestin) [Cleaved into: S-arrestin short form]
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-404
  • 氨基酸序列
    MKANKPAPNH VIFKKISRDK SVTIYLGKRD YIDHVERVEP VDGVVLVDPE LVKGKRVYVS LTCAFRYGQE DIDVMGLSFR RDLYFSQVQV FPPVGASGAT TRLQESLIKK LGANTYPFLL TFPDYLPCSV MLQPAPQDVG KSCGVDFEIK AFATHSTDVE EDKIPKKSSV RLLIRKVQHA PRDMGPQPRA EASWQFFMSD KPLRLAVSLS KEIYYHGEPI PVTVAVTNST EKTVKKIKVL VEQVTNVVLY SSDYYIKTVA AEEAQEKVPP NSSLTKTLTL VPLLANNRER RGIALDGKIK HEDTNLASST IIKEGIDKTV MGILVSYQIK VKLTVSGLLG ELTSSEVATE VPFRLMHPQP EDPDTAKESF QDENFVFEEF ARQNLKDAGE YKEEKTDQEA AMDE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to photoactivated, phosphorylated RHO and terminates RHO signaling via G-proteins by competing with G-proteins for the same binding site on RHO. May play a role in preventing light-dependent degeneration of retinal photoreceptor cells.
  • 基因功能参考文献：
    1. Data indicate molecular dynamics simulations and site-directed fluorescence experiments on arrestin-1 interactions with rhodopsin, showing that loops within the C-edge of arrestin function as a membrane anchor. PMID: 28220785
    2. Conformational changes are involved in the arrestin-rhodopsin binding interface through multiple docking modes. PMID: 24724832
    3. K2A mutations in arrestin-1, -2, and -3 significantly reduced their binding to active phosphorhodopsin. PMID: 22275358
    4. maintenance of low levels of the active monomer is the biological role of arrestin-1 self-association PMID: 21288033
    5. We hypothesize that, although arrestin requires at least a single Rho*P to bind the membrane, a single arrestin can actually interact with a pair of receptors PMID: 21169358
    6. variant form of arrestin-1 binds rod outer segment membranes PMID: 21176771
    7. Alpha-helix I plays an indirect role in receptor binding, likely keeping beta-strand I, which carries several phosphate-binding residues, in a position favorable for its interaction with receptor-attached phosphates. PMID: 19883657
    8. Models suggest that the phosphorylated carboxy-terminal region of rhodopsin, Rh(330-348), undergoes significant conformational changes and becomes structured upon binding to arrestin. PMID: 15111114
    9. arrestin and p44 bind differently to different phosphorylated rhodopsin species and that this may be due to a structural difference between p44's and arrestin's basal states. PMID: 16475829

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  • 亚细胞定位：
    [Isoform A]: Cell projection, cilium, photoreceptor outer segment. Membrane; Peripheral membrane protein.; [Isoform B]: Cell projection, cilium, photoreceptor outer segment. Membrane; Peripheral membrane protein.
  • 蛋白家族：
    Arrestin family
  • 组织特异性：
    Detected in retina, in rod photoreceptor cells (at protein level).
  • 数据库链接：

    KEGG: bta:280922

    STRING: 9913.ENSBTAP00000028633

    UniGene: Bt.32317