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  4. Recombinant Caenorhabditis elegans Protein glp-1 (glp-1), partial

Recombinant Caenorhabditis elegans Protein glp-1 (glp-1), partial

  • 货号：
    CSB-YP319558CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP319558CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP319558CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP319558CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP319558CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    glp-1
  • Uniprot No.：
  • 别名：
    glp-1; emb-33; F02A9.6Protein glp-1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential signaling protein which has a major role in germline and embryonic development; involved in cell fate decisions that require cell-cell interactions. Probable membrane-bound receptor for putative ligands lag-2 and apx-1. Upon ligand activation, and releasing from the cell membrane, the glp-1/Notch intracellular domain (NICD) probably forms a transcriptional activator complex with lag-1 and lag-3 and regulates expression of various genes; targets in the germline include lst-1 and sygl-1. Involved in the specification of the cell fates of the blastomeres, ABa and ABp. Proper signaling by glp-1 induces ABa descendants to produce anterior pharyngeal cells, and ABp descendants to adopt a different fate. Contributes to the establishment of the dorsal-ventral axis in early embryos. Required in postmitotic neurons in order to maintain the developmentally arrested larval state known as dauer, probably in response to lag-2. Regulates germ cell mitotic proliferation probably by regulating MAP kinase phosphatase lip-1 expression. Required for oocyte growth control. Plays a negative role in lifespan.
  • 基因功能参考文献：
    1. These results provide the first experimental evidence of an essential role for HSP90 in Notch signaling in development. PMID: 29507057
    2. The s find that Notch-dependent active transcription sites occur in a probabilistic fashion and, unexpectedly, do so in a steep gradient across the stem cell pool. PMID: 27705743
    3. Zygotically expressed MOM-2 (Wnt) is responsible for two distinct cell-cell interactions as the MS blastomere produces one-third of the body wall muscles. Both the inhibitory and the activating interactions require precise spatiotemporal expression of zygotic MOM-2, which is dependent upon two distinct GLP-1 Notch signals. PMID: 28049659
    4. Study suggests that GLP-1 signaling is directly responsible for driving all stages of proliferation in the wild-type germline, and loss of its activity causes differentiation without intervening, distinctly regulated transit-amplifying divisions. PMID: 26158953
    5. glp-1(ar202), a temperature-sensitive gain-of-function C. elegans NOTCH mutant, was employed to delineate NOTCH-driven tumor responses to radiotherapy. PMID: 26120834
    6. regulatory element is highly conserved in the paralogous glp-1 genomic environment, suggesting redundant developmental and physiological roles for the two Notch paralogs in the C. elegans nervous system. PMID: 23305862
    7. POS-1 regulates glp-1 mRNA translation by blocking access of other RNA-binding proteins to a key regulatory sequence PMID: 23034181
    8. The functional defect in glp-1(Pro) mutants occurs prior to or at the level of ligand interaction. PMID: 12586701
    9. the balance between POS-1 and SPN-4 controls the translation of maternal glp-1 mRNA PMID: 12702662
    10. Results suggest that TBX-37 and TBX-38 play a key role in distinguishing the outcomes of two sequential Glp-1/Notch-mediated interactions. PMID: 15056620
    11. Data support a model in which GLP-1 function in MSP-dependent oocyte growth is separable from its role in the proliferation versus meiotic entry decision. PMID: 19502484

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell projection, axon.; [glp-1/Notch intracellular domain]: Nucleus.
  • 组织特异性：
    Expressed in the distal mitotic region of the germ line. May be absent from the gonadal distal tip cell (DTC).
  • 数据库链接：

    KEGG: cel:CELE_F02A9.6

    STRING: 6239.F02A9.6

    UniGene: Cel.6196