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  4. Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha

Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha

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  • 中文名称：
    Recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha
  • 货号：
    CSB-EP888242CBG
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    ; Snaclec rhodocytin subunit alpha; Aggretin alpha chain; Rhodoaggretin subunit alpha
  • 种属：
    Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    31.8kDa
  • 表达区域：
    1-136aa
  • 氨基酸序列
    GLEDCDFGWSPYDQHCYQAFNEQKTWDEAEKFCRAQENGAHLASIESNGEADFVSWLISQKDELADEDYVWIGLRAQNKEQQCSSEWSDGSSVSYENLIDLHTKKCGALEKLTGFRKWVNYYCEQMHAFVCKLLPY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The full-length cDNA ORF of Calloselasma rhodostoma Snaclec rhodocytin subunit alpha was N-terminally tagged a 6xHis-SUMO and then expressed in the E.coli cells. The recombinant Calloselasma rhodostoma Snaclec rhodocytin subunit alpha thus obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, this protein migrated to an approximately 28 kDa molecular mass band on the SDS-PAGE gel. This recombinant Snaclec rhodocytin subunit alpha protein may be used to produce specific antibodies or in the studies of snaclec rhodocytin-mediated signal transduction.

    Snaclec rhodocytin is a disulfide‐linked heterodimeric C‐type lectin derived from Calloselasma rhodostoma and is made up of α‐subunits and β‐subunits. The interaction between snaclec rhodocytin and C‐type lectin‐like receptor 2 (CLEC-2) induces platelet aggregation. CLEC‐2 physiologically binding to podoplanin (PDPN), which is expressed on some tumor cell types, participates in tumor cell‐induced platelet aggregation and tumor metastasis. This may propose a possible source of anti‐CLEC‐2 drugs for both antiplatelet and antimetastasis therapy through rhodocytin modification.

  • Datasheet & COA：
    Please contact us to get it.

引用文献

产品评价

靶点详情

  • 功能：
    Elicits platelet aggregation by the binding to the C-type lectin domain family 1 member B (CLEC1B/CLEC2). Binding leads to tyrosine phosphorylation in the cytoplasmic tail of CLEC1B, which promotes the binding of spleen tyrosine kinase (Syk), subsequent activation of PLC-gamma-2, and platelet activation and aggregation. Binding to GPIbalpha (GP1BA) and alpha-2/beta-1 (ITGA2/ITGB1) may also induce aggregation, but this is controversial.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Snaclec family
  • 组织特异性：
    Expressed by the venom gland.