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Recombinant Calloselasma rhodostoma Thrombin-like enzyme ancrod

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  • 中文名称：
    Recombinant Calloselasma rhodostoma Thrombin-like enzyme ancrod
  • 货号：
    CSB-YP338742CBG
  • 规格：
    ￥2616
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Thrombin-like enzyme ancrod; SVTLE; EC 3.4.21.74; Fibrinogen-clotting enzyme; Snake venom serine protease; SVSP; Venombin A
  • 种属：
    Calloselasma rhodostoma (Malayan pit viper) (Agkistrodon rhodostoma)
  • 蛋白长度：
    Full Length
  • 来源：
    Yeast
  • 分子量：
    28.6kDa
  • 表达区域：
    1-234aa
  • 氨基酸序列
    VIGGDECNINEHRFLVAVYEGTNWTFICGGVLIHPEWVITAEHCARRRMNLVFGMHRKSEKFDDEQERYPKKRYFIRCNKTRTSWDEDIMLIRLNKPVNNSEHIAPLSLPSNPPIVGSDCRVMGWGSINRRIDVLSDEPRCANINLHNFTMCHGLFRKMPKKGRVLCAGDLRGRRDSCNSDSGGPLICNEELHGIVARGPNPCAQPNKPALYTSIYDYRDWVNNVIAGNATCSP
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The recombinant Calloselasma rhodostoma Thrombin-like enzyme ancrod (SVTLE) is a yeast-expressed (1-234aa) protein with N-terminal 6xHis tag. The purity is 90%+ measured by SDS-PAGE. The highly developed genetic system, ease of use, reduced time input, and costs have made Pichia Pastoris an attractive organism for the expression and production of recombinant proteins. So we choose the Yeast system to express this recombinant Thrombin-like enzyme ancrod (SVTLE) protein, which is able to carry specifically designed plasmids, and the plasmid used consists of restriction sites that can be used to insert the gene sequence of interest. Transformation of yeasts with the plasmid produces the desired protein and can be appropriately scaled up.

    Ancrod is a thrombin-like enzyme that induces rapid defibrinogenation in humans by splitting fibrinopeptide A from fibrinogen. Ancord was developed for the treatment of ischaemic stroke and myocardial infarction as well as deep-vein thrombosis. In addition to reducing fibrinogen, ancord also lowers blood viscosity, suppresses erythrocyte aggregation, indirectly stimulates thrombolysis, and possibly leads to vasodilatation. It also possesses a weak anticoagulant activity at high dosages.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

I hope you are having a nice day. I was wondering if you have any data regarding the bioactivity of CSB-EP338742CBG.

A:
Very nice to receive your inquiry. The protein we provide is full-length which is very likely to be active in theory, but we haven't validated yet.

靶点详情

  • 功能：
    Thrombin-like snake venom serine protease that acts as an anticoagulant. It cleaves fibrinogen (FGA) to split off the A-fibrinopeptides (A, AY and AP), but not the B-fibrinopeptide. The resulting fibrin polymers are imperfectly formed and much smaller in size (1 to 2 um long) than the fibrin polymers produced by the action of thrombin. These ancrod-induced microthrombi are friable, unstable, urea-soluble and have significantly degraded alpha chains. They do not cross-link to form thrombi. They are markedly susceptible to digestion by plasmin and are rapidly removed from circulation by either reticuloendothelial phagocytosis or normal fibrinolysis, or both. Anticoagulation through the removal of fibrinogen from the blood is rapid, occurring within hours following its administration. It does not activate plasminogen and does not degrade preformed, fully cross-linked thrombin fibrin. It also reduces the level of plasminogen activator inhibitor (PAI) and may stimulate the release of tissue plasminogen activator (PLAT) from the endothelium. The profibrinolytic effect of these 2 actions appears to be limited to local microthrombus degradation.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Snake venom subfamily
  • 组织特异性：
    Expressed by the venom gland.