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  4. Recombinant Chicken Serine/threonine-protein kinase Chk1 (CHEK1)

Recombinant Chicken Serine/threonine-protein kinase Chk1 (CHEK1)

  • 货号：
    CSB-YP806796CH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806796CH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806796CH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806796CH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806796CH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CHEK1; CHK1Serine/threonine-protein kinase Chk1; EC 2.7.11.1; CHK1 checkpoint homolog; Checkpoint kinase-1
  • 种属：
    Gallus gallus (Chicken)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-476
  • 氨基酸序列
    MAVPFVEDWD LVQTLGEGAY GEVQLAVNRR TEEAVAVKIV DMKRAADCPE NIKKEICINK MLNHENVVKF YGHRREGATQ YLFLEYCSGG ELFDRIEPDI GMPEPEAQRF FQQLIAGVVY LHSMGITHRD LKPENLLLDE RDNLKISDFG LATVFKHNGR ERLLNKMCGT LPYVAPELLR RPEFRAEPVD VWACGVVLTA MLAGELPWDQ PSDSCQEYSD WKERKTYLAP WRKIDSAPLA LLHKILTENP TARITIPDIK KDRWYCRPLK KGTKRGRVSS GGVTESPGAL PKHIRSDTDF SPVKSALGED KASYSTSQPE PGTGGALWDS STGSIDRLVQ GISFSQPACP EHMLLNSQLL GTPGSSQSPW QRLVRRMTRF FTKLDADGSY RSLRDVCEKM GYGWKQSCTN QVTISTTDRR NNKLIFKVNL LEMESRILVD FRLSKGDGLE FKRHFLKIKG KLSDVVSTQK VWLPPP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. This inhibits their activity through proteasomal degradation, nucleo-cytoplasmic shuttling and inhibition by proteins of the 13-3-3 family. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May promote DNA repair, regulate chromatin assembly and the transcription of genes that regulate cell-cycle progression. May also play a role in replication fork maintenance.
  • 基因功能参考文献：
    1. Pericentriolar material expansion depended on CHK1 kinase. Nuclear CHK1 activation after DNA damage mirrored PCM expansion, suggesting a PCM/damage feedback loop whereby CHK1 regulates pericentrin-dependent PCM expansion to control its own activation. PMID: 26165835
    2. Chk1 signalling causes centrosome amplification after ionizing radiation by upregulating Cdk2 activity through activating phosphorylation. PMID: 19838212
    3. These results suggest a mechanism through which Chk1 could protect against tumorigenesis through its role in spindle checkpoint signaling. PMID: 17276342
    4. Chk1-dependent centrosome amplification in response to DNA alkylation damage. PMID: 17404511
    5. Inhibition of checkpoint kinase 1 (Chk1) by RNA-mediated interference or drug treatment suppressed DNA-damage-induced centrosome amplification. PMID: 17468739
    6. Chk1 appears to help defend genomic integrity through effects on several other pathways, including Fanconi anemia proteins, the mitotic spindle, and transcription of cell cycle-related genes [review] PMID: 18424430
    7. Results show that S345 phosphorylation is impaired in Chk1 S317A and S366A mutants, suggesting that modification of adjacent SQ sites promotes this key regulatory event. PMID: 19421147

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  • 亚细胞定位：
    Nucleus. Chromosome. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    Protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily
  • 数据库链接：

    KEGG: gga:374260

    STRING: 9031.ENSGALP00000040314

    UniGene: Gga.4288