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  4. Recombinant Chicken Sonic hedgehog protein (SHH)

Recombinant Chicken Sonic hedgehog protein (SHH)

  • 货号：
    CSB-YP849568CH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP849568CH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP849568CH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP849568CH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP849568CH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    SHH; Sonic hedgehog protein; SHH; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhNC) [Cleaved into: Sonic hedgehog protein N-product; ShhN; Shh N-terminal processed signaling domains; ShhNp)]
  • 种属：
    Gallus gallus (Chicken)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-425
  • 氨基酸序列
    CGPG RGIGKRRHPK KLTPLAYKQF IPNVAEKTLG ASGRYEGKIT RNSERFKELT PNYNPDIIFK DEENTGADRL MTQRCKDKLN ALAISVMNQW PGVKLRVTEG WDEDGHHSEE SLHYEGRAVD ITTSDRDRSK YGMLARLAVE AGFDWVYYES KAHIHCSVKA ENSVAAKSGG CFPGSATVHL EHGGTKLVKD LSPGDRVLAA DADGRLLYSD FLTFLDRMDS SRKLFYVIET RQPRARLLLT AAHLLFVAPQ HNQSEATGST SGQALFASNV KPGQRVYVLG EGGQQLLPAS VHSVSLREEA SGAYAPLTAQ GTILINRVLA SCYAVIEEHS WAHWAFAPFR LAQGLLAALC PDGAIPTAAT TTTGIHWYSR LLYRIGSWVL DGDALHPLGM VAPAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.; The dually lipidated sonic hedgehog protein N-product (ShhNp) is a morphogen which is essential for a variety of patterning events during development. Induces ventral cell fate in the neural tube and somites. Involved in the patterning of the anterior-posterior axis of the developing limb bud. Essential for axon guidance. Binds to the patched (PTCH1) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTCH1 represses the constitutive signaling activity of SMO.
  • 基因功能参考文献：
    1. cAMP dependent protein kinase A (PKA) is a key intracellular factor mediating SHH signaling through regulation of GLI3 processing. PMID: 27720745
    2. propose a model in which positive and negative transcriptional regulatory networks accumulate their functions in the intersection area of their expression regions to provide a restricted spot for the ZPA (zone of polarizing activity), the source of morphogen, Shh. PMID: 28205287
    3. SHH determines the formation of spinal cord during the development of chicken embryo. PMID: 26454495
    4. An intrinsic cell cycle clock in polarizing region cells of the chick limb bud times the duration of Sonic hedgehog (Shh) expression, which encodes the morphogen specifying digit pattern across the antero-posterior axis (thumb to little finger). PMID: 25001275
    5. These data identify Notch signalling as a novel modulator of Shh signalling that acts mechanistically via regulation of ciliary localisation of key components of its transduction machinery. PMID: 25995356
    6. Sonic Hedgehog has a conserved role in tonotopic organization of the avian basilar papilla and mammalian cochlea PMID: 25775517
    7. A dynamic Shh expression pattern, regulated by SHH and BMP signaling, coordinates fusion of primordia in the amniote face. PMID: 25605783
    8. SHH promotes the survival of neural crest cells by limiting apoptosis induced by the dependence receptor CDON during branchial arch development. PMID: 25193697
    9. Increased SHH in the posterior Slk leg bud causes expression of anterior ectopic SHH and preaxial polydactyly. PMID: 24907417
    10. Our findings illustrate that limb development requires a sweet spot in the level and timing of pathway activation that allows for the Shh-dependent expansion of posterior progenitors without interfering with early prepatterning functions of Gli3/Gli3R. PMID: 24726283
    11. Sonic hedgehog signalling plays an important role in vertebrate development including ventral body wall formation. PMID: 23124188
    12. Shh signalling is required but the molecular network controlling cranial base development is distinct from that in the trunk PMID: 23009899
    13. Hh is an essential modulator of VEGF expression during developmental angiogenesis. PMID: 22513894
    14. Hoxd13 and Sall1 are syn-expressed in the posterior region of early chick wing buds together with 6 novel genes which are likely to be functionally related and represent secondary targets of Shh signalling. PMID: 20708683
    15. The majority of upper jaw shape variation can be explained by progressive changes in the spatial organization and mitotic activity of midfacial growth zones controlled by SHH signaling. PMID: 20826528
    16. Shh affects post-crossing axon guidance both directly and indirectly by regulating Wnt function. PMID: 20720124
    17. Data show that BMP4 and Shh constitute a dorsal-ventral signaling system together with FGF8. PMID: 20298259
    18. A balance between different pathways ensures the robustness of timely somite formation and notochord-derived Shh is a component of the molecular network regulating the pace of the somitogenesis clock. PMID: 20615943
    19. Within the rostral hindbrain, the Shh-activated homeodomain proteins Nkx2.2 and Nkx6.1 cooperate to induce the closely related zinc-finger transcription factors Gata2 and Gata3. PMID: 14973276
    20. Shh stimulates oligodendrocyte progenitor specification and simultaneously restricts the ventral extension of the astrocyte progenitor domain and reduces astrocyte development. PMID: 15183716
    21. Shh maintains bud development by localizing the dermal feather progenitors. PMID: 15242792
    22. Shh-expressing cells and their descendants cannot express Gremlin; proliferation of these descendants forms a barrier separating the Shh signal from Gremlin-expressing cells which breaks down the Shh-Fgf4 loop and affects limb size PMID: 15256670
    23. Shh is sileced in the right side of the Hensen's node by Pcl2 PMID: 15294861
    24. Hedgehog signaling has a role in vascular development PMID: 15294868
    25. Sonic hedgehog (Shh) and FGF8 have strong synergistic effects on chondrogenesis in vitro and promote outgrowth and chondrogenesis in vivo, suggesting a very specific role in producing elongated beak structures during chick facial development. PMID: 15302603
    26. Temporal perturbations in sonic hedgehog signaling elicit the spectrum of holoprosencephaly. PMID: 15314685
    27. the hedgehog (Hh) pathway plays a role in maintenance of the retina pigmented epithelium PMID: 15342484
    28. Overexpression of Shh in facial motor neurons of axotomized neonatal rats may promote neuronal survival. Adenovirus-mediated overexpression of chicken Shh transiently rescues axotomy-induced neuronal cell death for 3-5 d after axotomy. PMID: 15356205
    29. Data indicate that the rostral neural tube environment contains a signal different from both Sonic hedgehog and retinoic acid that acts on motoneuron differentiation. PMID: 15733664
    30. Sonic Hedgehog signaling plays a role in the patterning of the ventral neural tube PMID: 15741323
    31. Sonic hedgehog is necessary and sufficient for sine oculis-related homeobox 1 expression in posterior limb regions PMID: 15804569
    32. Thalamic development is induced by SHH in the chick embryo. PMID: 16026780
    33. miR-196 acts upstream of Hoxb8 and Sonic hedgehog (Shh) in vivo in the context of limb development, thereby identifying a previously observed but uncharacterized inhibitory activity that operates specifically in the hindlimb PMID: 16319892
    34. Data suggest that Sonic hedgehog (Shh) signaling regulates patterning, proliferation and survival of neural cells, thus Shh/Gli activity couples these separate cellular responses of progenitors to coordinate neural development. PMID: 16410413
    35. Shh contributes to CDC25B transcriptional activation in the neural tube both of chick and mouse embryos PMID: 16564519
    36. Results suggest that sonic hedgehog derived from the ventral foregut endoderm ensures the survival of neural crest cells at a critical stage of branchial arch development. PMID: 16868080
    37. Sonic hedgehog in the pharyngeal endoderm controls arch pattern via regulation of Fgf8 in head ectoderm. PMID: 17187772
    38. mechanisms showing how FGF2 and Shh signaling are interdependent during retina regeneration PMID: 17385725
    39. regulation between Fgf19 and the signaling molecules of the isthmic and zli organizers: Fgf8 and Shh, respectively. PMID: 17654705
    40. Shh and Fgf8 regulate each other's expression in this frontonasal organizer by either blocking or ectopically activating these pathways. PMID: 17988255
    41. Shh can regulate both cell number and the distribution of cell types in dorsal root gangia and plays an important role in the specification, patterning and pathfinding of sensory neurons. PMID: 18190905
    42. endoderm zone I ablation was rescued by Shh-loaded beads, whereas inhibition of Shh signalling suppressed mesethmoid formation PMID: 18539922
    43. Shh-producing cells create a zone of polarizing activity for the structures deriving from them PMID: 18539924
    44. Results indicate that the Smo-induced Galpha(i) activity plays an insignificant role in the regulation of Gli3 processing and Shh-regulated neural tube patterning. PMID: 18590719
    45. Shh continues to be expressed in tissues surrounding the prospective adenohypophysis. PMID: 18647663
    46. SHH is exclusively expressed in the conjunctival (scleral) papillae and not in the mesenchyme. PMID: 18855894
    47. The brain establishes multiple Sonic hedgehog (SHH) signaling centers within the developing upper jaw. PMID: 19036802
    48. Alteration of the extracellular environment through trypsin treatment can have profound effects on limb patterning. These effects appear to be mediated by the induction of Shh in host tissues and by ectopic apical ectodermal ridge formaion. PMID: 19207183
    49. tested whether reducing hedgehog signaling caused redeployment of the dorsal-ventral axis of the injured neural tube, allowing generation of new neural crest-like cells PMID: 19765571
    50. in hypertrophic chondrocytes, HS-assisted, TG-mediated Hh oligomerization modulates signaling via enhanced protein signaling activity PMID: 19801637

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  • 亚细胞定位：
    Endoplasmic reticulum membrane. Golgi apparatus membrane.; [Sonic hedgehog protein N-product]: Cell membrane; Lipid-anchor.
  • 蛋白家族：
    Hedgehog family
  • 组织特异性：
    Expressed in the posterior limb bud mesenchyme, the Hensen node, the notochord, and the floor plate of the neural tube.
  • 数据库链接：

    KEGG: gga:395615

    STRING: 9031.ENSGALP00000010292

    UniGene: Gga.345