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  4. Recombinant Chicken Triosephosphate isomerase (TPI1)

Recombinant Chicken Triosephosphate isomerase (TPI1)

  • 中文名称：
    鸡TPI1重组蛋白
  • 货号：
    CSB-YP024102CH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    鸡TPI1重组蛋白
  • 货号：
    CSB-EP024102CH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    鸡TPI1重组蛋白
  • 货号：
    CSB-EP024102CH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    鸡TPI1重组蛋白
  • 货号：
    CSB-BP024102CH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    鸡TPI1重组蛋白
  • 货号：
    CSB-MP024102CH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TPI1
  • Uniprot No.：
  • 别名：
    TPI1; Triosephosphate isomerase; TIM; EC 5.3.1.1; Methylglyoxal synthase; EC 4.2.3.3; Triose-phosphate isomerase
  • 种属：
    Gallus gallus (Chicken)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-248
  • 氨基酸序列
    APRKFFVGG NWKMNGDKKS LGELIHTLNG AKLSADTEVV CGAPSIYLDF ARQKLDAKIG VAAQNCYKVP KGAFTGEISP AMIKDIGAAW VILGHSERRH VFGESDELIG QKVAHALAEG LGVIACIGEK LDEREAGITE KVVFEQTKAI ADNVKDWSKV VLAYEPVWAI GTGKTATPQQ AQEVHEKLRG WLKSHVSDAV AQSTRIIYGG SVTGGNCKEL ASQHDVDGFL VGGASLKPEF VDIINAKH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis.; It is also responsible for the non-negligible production of methylglyoxal a reactive cytotoxic side-product that modifies and can alter proteins, DNA and lipids.
  • 基因功能参考文献：
    1. The hypothesis that limited access to conformational space is a requirement for protein hinges involved in catalysis has been successfully tested with glycine N- and C-terminal hinge mutants of loop 6 of triosephosphate isomerase (TIM). PMID: 15350130
    2. NMR study of wild-type (WT) TIM and a quintuple mutant (PGG/GGG). PMID: 17336327
    3. There is clear evidence for motion with an exchange rate constant consistent with opening and closing of triosephosphate isomerase active site loop (loop 6) being partially rate-limiting to catalytic throughput. PMID: 17455914
    4. 1st essential mode contributing to 34% of overall motion of dimer is strongly coupled to loop 6's closure over active site. Onset of collective motions at ns time scale due to dimerization has functional implications as to coordination of loop 6 closure. PMID: 18189421
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Triosephosphate isomerase family
  • 数据库链接：

    KEGG: gga:396435

    STRING: 9031.ENSGALP00000023396

    UniGene: Gga.4148