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  4. Recombinant Cowpea severe mosaic virus RNA2 polyprotein

Recombinant Cowpea severe mosaic virus RNA2 polyprotein

  • 货号：
    CSB-YP330100CRE
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP330100CRE
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP330100CRE-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP330100CRE
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP330100CRE
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    RNA2 polyprotein; Genome polyprotein M; M RNA polyprotein; Middle component RNA polyprotein; P2) [Cleaved into: VP58; P58); Movement protein; MP); Large capsid protein; LCP; Coat protein VP37; L subunit; Large coat protein); Small capsid protein precursor; S subunit); Mature small capsid protein; SCP; Coat protein VP23; Small capsid protein; N-terminus part; Small coat protein; N-terminus part); Small capsid protein C-terminus part; Small coat protein C-terminus part)]
  • 种属：
    Cowpea severe mosaic virus (strain DG) (CPSMV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-433
  • 氨基酸序列
    MSTFRYKCKQ LDQEIQWWFS GTGNRAFWKF EKKLAELHEW YWSLALDPFP YSGFFYKCFY ELFQLWVKLG LIVQVSYLIL LLDFFVYTIP KKMASQIETT VEKVKQSGIP ADILRKRAVD YWKKNNSHNS QMQDVLPNVD EIYEGMRANI AKYLGRSSTV TSIAKLGKCK VYRKKNIPLA NLPSLQTSCV PITLTEESVG NSDYTTEETN SEVKSLHVGA IEIVMNSFAS SDCNILGGFL LIDTCHTDIN NAIRSIFVAP MRGGRPIRMI SFPDTLVQIE PNMNKRFQLL CTTSNGDFMQ GRDLAMMHVN VLAHAVTHTS TYTPTPYYEK ILSREKGFIV EYLNRMTYAV HNQNHPTEKD LLESDFQFDF EGQPVLKRIS STKAIFSKGS SFRYMISGKK EHKIDKPRLE EDGSKSYIDG LQDTFDTTHA TLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responsible for viral RNA2 accumulation. May function by recruiting the RNA1-encoded polyprotein that contains the replication protein to RNA2 and enable its replication.; Transports the viral genome to neighboring plant cells directly through plasmosdesmata, without any budding. The movement protein allows efficient cell to cell propagation, by bypassing the host cell wall barrier. Acts by forming a tubular structure at the host plasmodesmata, enlarging it enough to allow free passage of virion capsids. Binds to GTP and to single-stranded RNA and single-stranded DNA in a non-sequence-specific manner.; Together with the mature small capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the small capsid protein. The large capsid protein interacts with the viral RNA.; Together with the large capsid protein, forms an icosahedral capsid (T=3) enclosing the viral positive strand RNA genome, with a diameter of approximately 300 Angstroms. The capsid is formed from 60 copies each of the large and the small capsid protein. The mature small capsid protein forms the turrets at the fivefold axes of the viral particle.; The cleavable C-terminus of small capsid protein seems to be involved in viral assembly and RNA packaging. After virus assembly, these amino acids are cleaved off during the normal maturation of the virus. Also seems to act as suppressor of post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.; Acts as a suppressor of RNA-mediated gene silencing, also known as post-transcriptional gene silencing (PTGS), a mechanism of plant viral defense that limits the accumulation of viral RNAs.
  • 亚细胞定位：
    [Movement protein]: Host cell junction, host plasmodesma.; [Large capsid protein]: Virion.; [Mature small capsid protein]: Virion.
  • 蛋白家族：
    Comoviridae genome polyprotein M family