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  4. Recombinant Cyclin-dependent kinase inhibitor 1 (cki-1)

Recombinant Cyclin-dependent kinase inhibitor 1 (cki-1)

  • 货号：
    CSB-YP631501CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP631501CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP631501CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP631501CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP631501CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    cki-1
  • Uniprot No.：
  • 别名：
    cki-1; T05A6.1Cyclin-dependent kinase inhibitor 1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-184
  • 氨基酸序列
    MSSARRCLFG RPTPEQRSRT RIWLEDAVKR MRQEESQKWG FDFELETPLP SSAGFVYEVI PENCVPEFYR TKVLTVRTTC SSLDISSTTL TPLSSPSTSD KEEPSLMDPN SSFEDEEEPK KWQFREPPTP RKTPTKRQQK MTDFMAVSRK KNSLSPNKLS PVNVIFTPKS RRPTIRTRSS CSPY
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Negative cell-cycle regulator that functions at the G1-to-S-phase transition. Required for suspension of the cell cycle in dauer larvae and starved L1 larvae. In vulval precursor cells (VPCs), a pathway of heterochronic genes acts via cki-1 to maintain VPCs in G1 during the L2 larval stage. Cul-2 may function in ubiquitin-mediated degradation by targeting cki-1 for degradation. Involved in distal tip cell development by repressing and modulating cye-1/cdk-2 activity levels in Z1.aa/Z4.pp and in Z1.ap/Z4.pa.
  • 基因功能参考文献：
    1. These data also suggest that integrin plays a major role in maintaining proper CKI-1/p27(KIP1) levels in the cell. PMID: 22879977
    2. BRAP-2 is necessary for preventing an inappropriate response to elevated levels of reactive oxygen species by countering premature activation of BRC-1 and CKI-1. PMID: 20207739
    3. CKI-1 cyclin-dependent kinase inhibitor has an essential embryonic role in cell cycle exit and morphogenesis. PMID: 12885569
    4. cki-1 is required to ensure the proper formation ans specification of the gonadal sheath cell precursors. PMID: 14597199
    5. Both cki-1 and cki-2 mediate cell cycle quiescence, but our genetic and phenotypic analyses demonstrate that they act within distinct pathways to exert control over the cell cycle machinery. PMID: 19597327

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    CDI family
  • 组织特异性：
    In embryos, expression is first seen in pharyngeal primordium and later in all differentiating cells. Post embryonic expression corresponds to developmental patterns of cell cycle progression in many tissues including sex myoblasts, distal tip cells, vulv
  • 数据库链接：

    KEGG: cel:CELE_T05A6.1

    STRING: 6239.T05A6.1

    UniGene: Cel.19541