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  4. Recombinant Cysteine protease avirulence protein AvrPphB (avrPph3)

Recombinant Cysteine protease avirulence protein AvrPphB (avrPph3)

  • 中文名称：
    Recombinant Cysteine protease avirulence protein AvrPphB(avrPph3),Yeast
  • 货号：
    CSB-YP709551FGN
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Cysteine protease avirulence protein AvrPphB(avrPph3),Yeast
  • 货号：
    CSB-EP709551FGN
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Cysteine protease avirulence protein AvrPphB(avrPph3),Yeast
  • 货号：
    CSB-EP709551FGN-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Cysteine protease avirulence protein AvrPphB(avrPph3),Yeast
  • 货号：
    CSB-BP709551FGN
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Cysteine protease avirulence protein AvrPphB(avrPph3),Yeast
  • 货号：
    CSB-MP709551FGN
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    avrPph3
  • Uniprot No.：
  • 别名：
    avrPph3; Cysteine protease avirulence protein AvrPphB; EC 3.4.22.-) [Cleaved into: 7 kDa product; 28 kDa product]
  • 种属：
    Pseudomonas syringae pv. phaseolicola
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-62
  • 氨基酸序列
    MKIGTQATSL AVLHNQESHA PQAPIAVRPE PAHAIPEIPL DLAIRPRTRG IHPFLAMTLG DK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cysteine protease avirulence protein, which is essential during infection of plant cells from cultivar-specific of beans and Arabidopsis thaliana. The autocleavage of the protein is required for virulence function. May act by affecting the plant defense system. In plants lacking R3 or RPS5 resistance genes, it probably impairs the plant defense system and leads to the bacteria multiplication. In contrast, in plants containing the R3 or RPS5 protein, it is unable to induce disease symptoms, explaining its avirulence name. The 7 kDa product is required for the type-III translocation from Pseudomonas strains to the plant, but are partially dispensable for effector recognition following in planta expression. In infected plants, it acts by cleaving the PBS1 protein, which leads to resistance or disease, depending on the presence or absence of RPS5, respectively. Targets the Arabidopsis kinases PBS1, BIK1, PBL1, PBL2, PBL3, PBL5, PBL7, PBL9 and PBL11 for cleavage in vitro. Can block recognition of AvrB avirulence factor by plant cells by cleaving Arabidopsis RIPK kinase and suppressing Arabidopsis RPM1 activation. Cannot block AvrRpm1-induced activation of RPM1.
  • 亚细胞定位：
    Secreted. Host membrane. Note=In infected plant cells, it is membrane-associated.
  • 蛋白家族：
    Peptidase C58 family
  • 数据库链接：

    KEGG: ag:AAA25727