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  4. Recombinant Deinococcus radiodurans Single-stranded DNA-binding protein (ssb)

Recombinant Deinococcus radiodurans Single-stranded DNA-binding protein (ssb)

  • 中文名称：
    放射性红球菌ssb重组蛋白
  • 货号：
    CSB-YP886576DJA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    放射性红球菌ssb重组蛋白
  • 货号：
    CSB-EP886576DJA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    放射性红球菌ssb重组蛋白
  • 货号：
    CSB-EP886576DJA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    放射性红球菌ssb重组蛋白
  • 货号：
    CSB-BP886576DJA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    放射性红球菌ssb重组蛋白
  • 货号：
    CSB-MP886576DJA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ssb
  • Uniprot No.：
  • 别名：
    ssb; DR_0099; Single-stranded DNA-binding protein; SSB
  • 种属：
    Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-301
  • 氨基酸序列
    MARGMNHVYL IGALARDPEL RYTGNGMAVF EATVAGEDRV IGNDGRERNL PWYHRVSILG KPAEWQAERN LKGGDAVVVE GTLEYRQWEA PEGGKRSAVN VKALRMEQLG TQPELIQDAG GGVRMSGAMN EVLVLGNVTR DPEIRYTPAG DAVLSLSIAV NENYQDRQGQ RQEKVHYIDA TLWRDLAENM KELRKGDPVM IMGRLVNEGW TDKDGNKRNS TRVEATRVEA LARGAGNANS GYAAATPAAP RTQTASSAAR PTSGGYQSQP SRAANTGSRS GGLDIDQGLD DFPPEEDDLP F
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism. Essential for ionizing radiation resistance. Stimulates the 5'-3' DNA helicase activity of RecD-like helicase. Stimulates RecA protein-promoted DNA three-strand exchange reactions in vitro with both D.radiodurans and E.coli-derived RecA. Complements an ssb deletion in E.coli, but does not complement a ddrb disruption in D.radiodurans.
  • 基因功能参考文献：
    1. The gene product of drb009 is involved in the DNA repair. PMID: 18338577
  • 亚细胞定位：
    Cytoplasm. Note=During log phase, present in multiple foci. 1 hour after irradiation most foci are condensed in the center of the cell's nucleoid, they dissipate by 2 hours later.
  • 数据库链接：

    KEGG: dra:DR_0099

    STRING: 243230.DR_0100