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  4. Recombinant Dictyostelium discoideum Glycogen synthase kinase-3 (gskA)

Recombinant Dictyostelium discoideum Glycogen synthase kinase-3 (gskA)

  • 货号：
    CSB-YP344691DKK
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP344691DKK
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP344691DKK-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP344691DKK
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP344691DKK
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    gskA
  • Uniprot No.：
  • 别名：
    gskA; gsk3; DDB_G0272110; Glycogen synthase kinase-3; GSK-3; EC 2.7.11.26
  • 种属：
    Dictyostelium discoideum (Slime mold)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-467
  • 氨基酸序列
    MSSKDQILEK DKKETDDNGN KKTTTTTSSS SSSSSSSKPR SNKFDKVIIK SNGVCYITEG VIGNGSFGVV TQAIVADTKE VVAIKKVLQD QRYKNRELQI MKMLNHINIV SLKNSFYTSD NDEVYLNLVL EYVPDTVYRV SRHYSMSKQP VPNIFVKLYI YQLCRSINYI HSLGICHRDI KPQNLLLDTS TSTLKLCDFG SAKILIKGET NVSYICSRHY RAPELIFGST NYTTTIDVWS LGCVLAELLL GQPLFPGENG IDQLVEIIKV LGTPTKEQIH AMNPYYTSFK FPEIKANPWP RVFKAKDVPA ESIDLISKIL LYDPSSRLKP VEICAHPFFD ELRDPKTCLP DGKPLPPLFN FTIAEQTSIG PKLAKTLIPS HAMNQIELPS PLFPNLAISS SNQSSSSNSN ANVSSNLNSH SASPSTTSSS SSTPNSIPVQ SPSTTNTTSS TTNNTTTTTT TTTTSNH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    During cellular differentiation, may mediate an extracellular cyclic AMP stimulated signal transduction pathway that regulates prespore and prestalk B-cell proportions through inhibition of stalk cell formation and induction of prespore cell differentiation. The cAMP receptor carC appears to activate gskA via the tyrosine kinases zakA and zak2, to stimulate prespore differentiation, while carD appears to negatively regulate gskA, to promote prestalk formation.
  • 基因功能参考文献：
    1. gskA-null mutants failed to elongate their mitotic spindle and were unable to divide, but have no chromosome segregation defect. PMID: 23787121
    2. GSK-3 function is essential for chemotaxis, regulating multiple substrates, and one of these effectors, DydA, plays a key function in the dynamic regulation of chemotaxis. PMID: 23135995
    3. Cells lacking gskA exhibit poor chemotaxis toward cAMP and folate. PMID: 20534815
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, GSK-3 subfamily
  • 数据库链接：

    KEGG: ddi:DDB_G0272110

    STRING: 44689.DDB0185150