Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Dog Insulin (INS), partial

Recombinant Dog Insulin (INS), partial

  • 货号：
    CSB-YP011742DO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011742DO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011742DO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011742DO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011742DO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    INS; Insulin [Cleaved into: Insulin B chain; Insulin A chain]
  • 种属：
    Canis familiaris (Dog) (Canis lupus familiaris)
  • 蛋白长度：
    partial
  • 表达区域：
    25-54
  • 氨基酸序列
    FVNQHL CGSHLVEALY LVCGERGFFY TPKA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

A few questions :
1. Is this the beta-chain? Seems so when comparing the amino acid sequence.
2. Could you estimate if the tag removal can be successful?
3. If it is not the full length insulin, is it possible to get the full length insulin?
I am intend to do mass-spec for these proteins. Is there any recommendation that which expression system will be best for this application?

A:
Very nice to receive your inquiry.
1) Yes. This is Insulin B chain.
2) We could try to remove the tag. The previous success rate of our tag removal service is 75%-86%.
Now we could simultaneously connect multiple carriers for tag removal and the success rate will be higher than previous. If we succeed in removing the tag, we will charge for extra cost.
The lead time for tag removal service will be 3 working days.
3) If the customer just need conventional mass spectrometry identification, we could perform MS for all the four expression systems.

靶点详情

  • 功能：
    Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
  • 基因功能参考文献：
    1. The etiology of blindness in dogs with pituitary dependent hypercortisolism, including the role of adiponectin, interleukin 6, insulin, and nitric oxide, is reported. PMID: 22381588
    2. Gluconeogenic flux can be rapidly inhibited by high insulin levels in canines. PMID: 19755527
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    KEGG: cfa:483665

    STRING: 9615.ENSCAFP00000040273