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  4. Recombinant Drosophila melanogaster Apoptosis 1 inhibitor (th)

Recombinant Drosophila melanogaster Apoptosis 1 inhibitor (th)

  • 中文名称：
    黑腹果蝇Diap1重组蛋白
  • 货号：
    CSB-YP631540DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Diap1重组蛋白
  • 货号：
    CSB-EP631540DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Diap1重组蛋白
  • 货号：
    CSB-EP631540DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Diap1重组蛋白
  • 货号：
    CSB-BP631540DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Diap1重组蛋白
  • 货号：
    CSB-MP631540DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Diap1
  • Uniprot No.：
  • 别名：
    Diap1; Iap1; th; CG12284Death-associated inhibitor of apoptosis 1; EC 2.3.2.27; Apoptosis 1 inhibitor; E3 ubiquitin-protein ligase th; Inhibitor of apoptosis 1; Protein thread; RING-type E3 ubiquitin transferase Diap1
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-438
  • 氨基酸序列
    MASVVADLPS YGPIAFDQVD NNTNATQLFK NNINKTRMND LNREETRLKT FTDWPLDWLD KRQLAQTGMY FTHAGDKVKC FFCGVEIGCW EQEDQPVPEH QRWSPNCPLL RRRTTNNVPI NAEALDRILP PISYDICGAN DSTLEMREHA YAEGVIPMSQ LIQSIGMNAV NAAGSVTGTA APQPRVTVAT HASTATQATG DVQPETCRPS AASGNYFPQY PEYAIETARL RTFEAWPRNL KQKPHQLAEA GFFYTGVGDR VRCFSCGGGL MDWNDNDEPW EQHALWLSQC RFVKLMKGQL YIDTVAAKPV LAEEKEESSS IGGVAVASTQ ASEEEQQTSL SSEEAVSGDV APSVAPTAAT RIFNKIVEAT AVATPSTNSS GSTSIPEEKL CKICYGAEYN TAFLPCGHVV ACAKCASSVT KCPLCRKPFT DVMRVYFS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Anti-apoptotic protein which functions as a caspase regulator, using its E3 ubiquitin-protein ligase activity to smother caspase activity. Binds, ubiquitinates and inactivates initiator caspase Dronc, and effector caspases Drice and Dcp-1. Acts as a Nedd8-E3 ubiquitin-protein ligase for Drice. Suppresses apoptosis by targeting the apoptosome for ubiquitination and inactivation. Plays an important role in cell motility. Overexpression suppresses rpr and hid-dependent cell death in the eye. Interaction of Diap1 with Dronc is required to suppress Dronc-mediated cell death through Diap1-mediated ubiquitination of Dronc. Acts as a positive regulator of Wnt signaling.
  • 基因功能参考文献：
    1. Both germline and somatic stem cells in the adult Drosophila testis are more resistant to apoptosis-inducing stimuli than their differentiating daughters. Niche signaling causes enhanced expression of DIAP1 in both germline stem cells and somatic cyst stem cells, and the resistance to apoptosis is mediated, at least in part, by this enhanced expression. PMID: 25941003
    2. DIAP1 contains distinct instability motifs that regulate inhibitor-of-apoptosis turnover and apoptotic consequences. PMID: 25653450
    3. This study showed that Diap2 could be regulating ion channels, as loss of Diap2 consequently reduces the expression of ion channels and affects the balance of ion concentrations which results in reduced uric acid deposition. PMID: 24374974
    4. DIAP1 is an important survival factor that inhibits premature death of CCAP-expressing neurons. PMID: 23784845
    5. dBruce inhibits apoptosis by promoting IAP-antagonist ubiquitination on unconventional acceptor sites. PMID: 21886178
    6. Drosophila CtBP (dCtBP) was identified as a binding partner of Diap1; whether the interaction between Diap1 and dCtBP also occurs in human cells was tested. PMID: 22138398
    7. DIAP1-mediated ubiquitylation does not trigger proteasomal degradation of full-length DRONC, but serves a non-proteolytic function PMID: 21909282
    8. The cleaved DIAP1 binds drICE, inhibits its protease activity, and also targets drICE for ubiquitylation. PMID: 21811237
    9. host pathway initiated by virus DNA replication and acting through instability motifs within IAP triggers IAP depletion and causes apoptosis; suggests host modulation of IAP levels is mechanism by which insects mount an apoptotic antiviral response PMID: 21653668
    10. the anti-apoptotic factor drosophila inhibitor of apoptosis 1 protein is downregulated in these cells in a head involution defect protein-dependent manner PMID: 21113144
    11. VCP binds to DIAP1 in a ubiquitin- and BIR domain-dependent manner and facilitates its degradation, therefore establishing a new link between ubiquitin, dendrite pruning and the apoptosis machinery. PMID: 21343367
    12. Results suggest that the hsromega transcripts regulate cellular levels of DIAP1 via the hnRNP Hrb57A, which physically interacts with DIAP1. PMID: 19737742
    13. the existence of a novel mechanism for cell death signaling in Drosophila that is mediated by DRONC and Dark. PMID: 12011068
    14. Hid, Rpr and Grim negatively regulate DIAP1 levels through distinct mechanisms PMID: 12021767
    15. Regulation of Drosophila IAP1 degradation and apoptosis by reaper and ubcD1. PMID: 12021769
    16. Jafrac2 is an IAP antagonist that promotes cell death by liberating Dronc from this protein PMID: 12356728
    17. DIAP1 is required to prevent excess accumulation of the first form of processed DRONC, presumably through its ability to act as a ubiquitin-protein ligase PMID: 12397080
    18. DIAP1 is degraded by the 'N-end rule' pathway and this process is indispensable for regulating apoptosis. Caspase-mediated cleavage of DIAP1 at position 20 converts DIAP1 into an unstable, Asn-bearing, DIAP1 N-degron of the N-end rule degradation pathway. PMID: 12692559
    19. Overexpression of Diap1 suppressed cell death induced by dcp-1 but had no effect on cell death during late oogenesis. PMID: 12885559
    20. data strongly suggest that inhibitors of apoptosis 1 destabilization by Reaper in intact cells requires localization to mitochondria and that induction of this instability is important for the potent induction of apoptosis in Drosophila cells PMID: 12917412
    21. Overexpression of IAP1 suppressed cell migration and did not cause apoptosis. PMID: 15242648
    22. model for DIAP1 function in living and dying cells PMID: 15371434
    23. DIAP1-sequestered effector caspases remain catalytically active, suggesting that DIAP1 does not function as a bona fide enzyme inhibitor PMID: 15580265
    24. IKK epsilon function involves the negative regulation of the nonapoptotic activity of DIAP1. PMID: 16887350
    25. dOmi alleviates Drosophila inhibitor of apoptosis 1 inhibition of all caspases by proteolytically degrading Drosophila inhibitor of apoptosis 1 and induces apoptosis both in cultured cells and in the developing fly eye PMID: 17557079
    26. findings suggest that DIAP1 is a critical sensor of RNA virus infection, which upon virus-signaled depletion relieves caspase inhibition, which subsequently executes apoptotic death. PMID: 17989181
    27. Data show that DIAP1 auto-ubiquitination and degradation was contributed by both Reaper binding and mitochondrial localization. PMID: 17998202
    28. our results suggest that the level of eIF4E protein is regulated by Diap1, and that IAPs may play a role in cap-dependent translation by regulating the level of eIF4E protein. PMID: 18182863
    29. The binding of DIAP1 to dOmi resulted in DIAP1-mediated polyubiquitination of dOmi, suggesting that DIAP1 could target dOmi for proteasomal degradation. PMID: 18259196
    30. Because DIAP1 prevents apoptosis after a variety of stresses, STAT92E may have a role in regulating stress responses in general PMID: 18779571
    31. suggest that HOW(L) enhances the sensitivity of MG cells to apoptotic signals by reducing the levels of diap1 in these cells in, demonstrating a novel mode of regulation of PCD at the mRNA level PMID: 18984040
    32. found that the quantity of DIAP1 changed dramatically depending on the cell type and maturity. PMID: 19822670

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  • 蛋白家族：
    IAP family
  • 数据库链接：

    KEGG: dme:Dmel_CG12284

    STRING: 7227.FBpp0305795

    UniGene: Dm.6466