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  4. Recombinant Drosophila melanogaster Caspase Nc (Nc)

Recombinant Drosophila melanogaster Caspase Nc (Nc)

  • 中文名称：
    黑腹果蝇Dronc重组蛋白
  • 货号：
    CSB-YP894569DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Dronc重组蛋白
  • 货号：
    CSB-EP894569DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Dronc重组蛋白
  • 货号：
    CSB-EP894569DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Dronc重组蛋白
  • 货号：
    CSB-BP894569DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Dronc重组蛋白
  • 货号：
    CSB-MP894569DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dronc
  • Uniprot No.：
  • 别名：
    Dronc; Nc; CG8091Caspase Dronc; EC 3.4.22.-; NEDD2-like caspase) [Cleaved into: Caspase Nc subunit 1; Caspase Nc subunit 2]
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    135-320
  • 氨基酸序列
    DTPSPE ASEGPCVSKL RNEPLGALTP YVGVVDGPEV KKSKKIHGGD SAILGTYKMQ SRFNRGVLLM VNIMDYPDQN RRRIGAEKDS KSLIHLFQEL NFTIFPYGNV NQDQFFKLLT MVTSSSYVQN TECFVMVLMT HGNSVEGKEK VEFCDGSVVD MQKIKDHFQT AKCPYLVNKP KVLMFPFCRG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in the activation cascade of caspases responsible for apoptosis execution. Effector of steroid-mediated apoptosis during insect metamorphosis. Overexpression promotes programmed cell death. Interaction with Diap1 is required to suppress Dronc-mediated cell death; via Diap1-mediated ubiquitination of Dronc. Rate-limiting caspase in rpr and hid death pathway.
  • 基因功能参考文献：
    1. Dronc plays a dual role that can either initiate DNA damage response or apoptosis. PMID: 28751499
    2. Dronc protein fails to accumulate in epithelial cells with impaired proteasome function suggesting that it is not degraded by the proteasome. PMID: 27104928
    3. e provide evidence that not all of the non-apoptotic functions of Dronc require its catalytic activity. In conclusion, we demonstrate a mechanism whereby Dronc's apoptotic and non-apoptotic activities can be kept silenced in a non-degradative manner through a single ubiquitylation event in living cells. PMID: 28207763
    4. the principal caspase required for the cleavage of Inhibitor of Apoptosis 2 Protein PMID: 25968339
    5. Findings suggest that the intrinsic properties of initiator caspase Dronc proteinsare responsible, at least in part, for the differing sensitivity of Ld652Y and BM-N to apoptosis induction upon nucleopolyhedroviruses (NPVs) infection. PMID: 23743202
    6. that DRONC protein accumulates in "undead" cells due to increased transcription of dronc in these cells PMID: 21909282
    7. Data suggest that the cleaved-Caspase-3 antibody recognizes multiple proteins including DCP-1 and likely DRICE, but also at least one additional unknown protein, all of which require DRONC for epitope exposure. PMID: 19960024
    8. mutations in Dronc, the Drosophila ortholog of initiator caspase-9, attenuated dendritic pruning more efficiently than inhibition of the effector caspases, transgenic expression of WldS, or both, indicating additional roles of Dronc in this process PMID: 20445064
    9. Cell death stimulated by POSH is dependent on the caspase-9 homolog, Drosophila Nc. PMID: 20014406
    10. a novel cell death pathway triggered by DIAP1 depletion depends on Dark and Dronc PMID: 12011068
    11. The DIAP1 RING finger mediates ubiquitination of Dronc and is indispensable for regulating apoptosis. PMID: 12021771
    12. DRONC caspase is regulated by ecdysone during apoptosis in Drosophila. The BR-C transcription factor regulates ecdysone during metamorphosis. PMID: 12045184
    13. Jafrac2 displaces Dronc from DIAP1 by competing with Dronc for the binding of DIAP1, consistent with the idea that Jafrac2 triggers cell death by liberating Dronc from DIAP1-mediated inhibition PMID: 12356728
    14. the initial step in DRONC processing occurs continuously via a DARK-dependent mechanism in Drosophila cells and DIAP1 is required to prevent excess accumulation of this first form of processed DRONC PMID: 12397080
    15. the dronc promoter is regulated in a highly complex manner PMID: 12970673
    16. Direct binding of EcR-Usp is crucial for controlling the timing of dronc expression in specific tissues. PMID: 15173191
    17. analysis of Dronc activation and processing in Drosophila cells PMID: 15383617
    18. dronc has a role in regulating programmed and unprogrammed cell death in Drosophila PMID: 15572131
    19. DRONC is involved in specific developmental cell death pathways PMID: 15572132
    20. DIAP1 is cleaved by the apical caspase DRONC in both normal and apoptotic Drosophila cells PMID: 15774476
    21. Our study places DRONC at a central point of convergence for multiple cell death pathways and for the ecdysone pathway regulating metamorphosis PMID: 15922568
    22. [extra views]Insights gained from the dronc mutants will help in advancing our understanding of caspase function in various developmental pathways. PMID: 15970694
    23. These results show that the apoptotic pathway bifurcates at DRONC and that DRONC coordinates the execution of cell death and compensatory proliferation. PMID: 16980627
    24. Results describe the activation mechanism and substrate specificity of the Drosophila initiator caspase DRONC. PMID: 18309328

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Peptidase C14A family
  • 组织特异性：
    Ubiquitously expressed in embryos during early stages of development. In late third instar larvae, dramatic up-regulation in salivary glands and midgut before histolysis of these tissues.
  • 数据库链接：

    KEGG: dme:Dmel_CG8091

    STRING: 7227.FBpp0076076

    UniGene: Dm.3230