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  4. Recombinant Drosophila melanogaster Polycomb protein esc (esc)

Recombinant Drosophila melanogaster Polycomb protein esc (esc)

  • 中文名称：
    黑腹果蝇esc重组蛋白
  • 货号：
    CSB-YP635523DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇esc重组蛋白
  • 货号：
    CSB-EP635523DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇esc重组蛋白
  • 货号：
    CSB-EP635523DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇esc重组蛋白
  • 货号：
    CSB-BP635523DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇esc重组蛋白
  • 货号：
    CSB-MP635523DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    esc
  • Uniprot No.：
  • 别名：
    esc; CG14941Polycomb protein esc; Protein extra sex combs
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-425
  • 氨基酸序列
    MSSDKVKNGN EPEESEESCG DESASYTTNS TTSRSKSPSS STRSKRRGRR STKSKPKSRA AYKYDTHVKE NHGANIFGVA FNTLLGKDEP QVFATAGSNR VTVYECPRQG GMQLLHCYAD PDPDEVFYTC AWSYDLKTSS PLLAAAGYRG VIRVIDVEQN EAVGNYIGHG QAINELKFHP HKLQLLLSGS KDHAIRLWNI QSHVCIAILG GVEGHRDEVL SIDFNMRGDR IVSSGMDHSL KLWCLNTPEF HHKIELSNTF SQEKSTLPFP TVTKHFPDFS TRDIHRNYVD CVQWFGNFVL SKSCENAIVC WKPGQLHQSF EQVKPSDSSC TIIAEFEYDE CEIWFVRFGF NPWQKVIALG NQQGKVYVWE LDPSDPEGAH MTTLHNSRSV ATVRQIAFSR DASVLVYVCD DATVWRWNRR QTTSI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Polycomb group (PcG) protein. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. Component of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3, leading to transcriptional repression of the affected target gene. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required.
  • 基因功能参考文献：
    1. The ESC is a key component of the Polycomb repressive complex 2 (PRC2), which mediates H3K27me3 modification. PMID: 21700228
    2. Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal Polycomb sites. PMID: 12408863
    3. We purified an ESC-E(Z) complex from Drosophila embryos and found four major subunits: ESC, E(Z), NURF-55, and the PcG repressor, SU(Z)12. A recombinant complex reconstituted from these four subunits methylates lysine-27 of histone H3. PMID: 12408864
    4. ESC and E(Z) are present in multiple distinct complexes that undergo dynamic changes during development PMID: 12533794
    5. Here, we show that Sex combs extra (Sce) encodes Ring and that Sce/Ring function is critically required for PcG silencing. PMID: 12963114
    6. extreme Polycomb embryonic phenotype due to the lack of maternal and zygotic Sex combs extra can be rescued by ectopic expression of Ring/Sex combs extra PMID: 15147763
    7. Thus, stability and perhaps assembly of larger ESC complexes may depend on ESC phosphorylation. PMID: 15896722
    8. esc and E(Pc) mutants enhance homologous recombination by modulating the histone acetylation status of histone H4 at the double-strand break PMID: 16452150
    9. one of the roles of ESC (and ESCL and EED) in Polycomb repressive complex 2 complexes is to enable Enhancer of zeste to utilize histone H3 as a substrate by physically linking enzyme and substrate PMID: 17210640
    10. A Pcl-PSC2 complex is the histone methyltransferase that generates the high levels of histone3-lysine27 trimethylation in Polycomb target genes that are needed to maintain a Polycomb-repressed chromatin state. PMID: 17762866
    11. These data thus indicate that ESC and ESCL play similar if not identical functions in E(Z) complexes in vivo. PMID: 18048023
    12. analysis of the roles of ESC and ESCL in Polycomb Group mechanisms PMID: 18276122

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    WD repeat ESC family
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: dme:Dmel_CG14941

    STRING: 7227.FBpp0079907

    UniGene: Dm.1948