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  4. Recombinant Drosophila melanogaster Stress-activated protein kinase JNK (bsk)

Recombinant Drosophila melanogaster Stress-activated protein kinase JNK (bsk)

  • 中文名称：
    Recombinant Drosophila melanogaster Stress-activated protein kinase JNK(bsk)
  • 货号：
    CSB-EP310094DLU
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    bsk
  • Uniprot No.：
  • 别名：
    bsk; JNK; CG5680; Stress-activated protein kinase JNK; dJNK; EC 2.7.11.24; Protein basket
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    47.0kDa
  • 表达区域：
    1-372aa
  • 氨基酸序列
    MTTAQHQHYTVEVGDTNFTIHSRYINLRPIGSGAQGIVCAAYDTITQQNVAIKKLSRPFQNVTHAKRAYREFKLMKLVNHKNIIGLLNAFTPQRNLEEFQDVYLVMELMDANLCQVIQMDLDHDRMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKADCTLKILDFGLARTAGTTFMMTPYVVTRYYRAPEVILGMGYTENVDIWSVGCIMGEMIRGGVLFPGTDHIDQWNKIIEQLGTPSPSFMQRLQPTVRNYVENRPRYTGYSFDRLFPDGLFPNDNNQNSRRKASDARNLLSKMLVIDPEQRISVDEALKHEYINVWYDAEEVDAPAPEPYDHSVDEREHTVEQWKELIYEEVMDYEAHNTNNRTR
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Responds to activation by environmental stress by phosphorylating a number of transcription factors, primarily components of AP-1 such as Jra and also the transcriptional repressor aop, and thus regulates transcriptional activity. Component of the immune response activated by bacterial infection, and is involved in wound healing and in dorsal closure, a morphogenetic movement during embryogenesis. Functions in the systematic response to wounding acting downstream of the Hayan-phenoloxidase PPO1 cascade. Exhibits cytoprotective activity in neuronal cells in response to wounding to the integument. Controls the expression of a phosphatase, puckered, at the edges of wounded epidermal tissue and in the dorsal epithelium during dorsal closure. Regulates the activity of SREBP in neurons and thereby the accumulation of lipids in glia. Plays a role in postively regulating the expression of DIP2 independently of AP-1, thereby ensuring proper axon guidance in mushroom bodies.
  • 基因功能参考文献：
    1. Increased expression of the Drosophila JNK basket in the setting of reduced cindr expression was found to result in even more severe apoptosis, whilst ectopic death was found to be reduced if retinas were heterozygous for basket. PMID: 29133184
    2. n addition to significantly increasing the number of JNK target genes identified so far, our results reveal that the LE is a highly heterogeneous morphogenetic organizer, sculpted through crosstalk between JNK, segmental and AP signalling. This fine-tuning regulatory mechanism is essential to coordinate morphogenesis and dynamics of tissue sealing PMID: 28231245
    3. Diminished MTORC1-dependent JNK activation underlies the neurodevelopmental defects associated with lysosomal dysfunction. PMID: 26387958
    4. This study demonstrated that the mechanism by which Bsk is required for pruning is through reducing the membrane levels of the adhesion molecule Fasciclin II (FasII) PMID: 26586184
    5. Study solves the crystal structure of unphosphorylated DJNK in complex with adenylyl imidodiphosphate (AMP-PNP) and magnesium. PMID: 26377800
    6. In a genetic screen, we identified signaling by the EGFR pathway as important for apoptosis-induced proliferation acting downstream of JNK signaling PMID: 24497843
    7. We propose that JNK is a central stress response effector that is activated by nucleolar stress in Drosophila larvae PMID: 23412656
    8. dUev1a modulates TNF-JNK mediated tumor progression and cell death in Drosophila. PMID: 23726905
    9. Raw negatively regulates JNK signaling during gonad morphogenesis, and increased JNK signaling is sufficient to cause ensheathment defects. PMID: 22575490
    10. ectopic Dpp led to DJNK-dependent cardial apoptosis through the non-canonical TGF-beta pathway during late embryogenesis of Drosophila. PMID: 22114909
    11. stress-induced apoptosis in Drosophila is dependant on an amplification loop mediated by dp53 and JNK PMID: 21886179
    12. Pan-neuronal knockdown of JNK mRNA expression by RNA interference resulted in a decrease in both sleep and longevity. PMID: 22197814
    13. Data show that regeneration of genetically ablated tissue requires JNK activity, and that the JNK pathway is activated at the leading edges of healing tissue and not in apoptotic cells, and is required for regulation of healing and regenerative growth. PMID: 20215351
    14. These findings demonstrate that Eiger can initiate cell death through an IAP-sensitive cell death pathway via JNK signaling. PMID: 12065414
    15. Results introduce the Jun N-terminal kinase (JNK) signaling pathway as a genetic determinant of aging in Drosophila melanogaster. PMID: 14602080
    16. Relish limits the duration of JNK activation in response to Gram-negative infections. Degradation of TAK1 leads to a rapid termination of JNK signaling, resulting in a transient JNK-dependent response that precedes sustained induction of immune loci. PMID: 15037551
    17. The convergence of JNK signaling and IIS on Foxo provides a model to explain the effects of stress and nutrition on longevity. PMID: 15820683
    18. JNK acts as the downstream mediator of the LKB1-dependent apoptosis. PMID: 16273080
    19. Study found that the left-right asymmetric cell rearrangement in the circular visceral muscle was regulated by D-JNK signaling and required for the LR asymmetric looping of the anterior-midgut. PMID: 17915206
    20. regulators of JNK were identified; using genetic & phosphoproteomics data, an integrative network algorithm to construct a JNK phosphorylation network was implemented PMID: 18927396
    21. influences regeneration in the gut by directing proliferation of intestinal stem cells, contributes to the loss of tissue homeostasis in old and stressed intestines PMID: 18940735
    22. JNK activity upregulates genes involved in intracellular transport in order to provide an increased level of trafficking activity in cells undergoing complex morphogenetic arrangements such as dorsal closure. PMID: 19427848

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
  • 组织特异性：
    During gastrulation, expression is seen in cells undergoing morphogenetic movements. By stage 9 of embryonic development, expression is ubiquitous. At stages 12-14, expression occurs in epidermis and central nervous system. At stage 15, expression is rest
  • 数据库链接：

    KEGG: dme:Dmel_CG5680

    STRING: 7227.FBpp0079676

    UniGene: Dm.1448