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  4. Recombinant Drosophila melanogaster Transcription initiation factor IIA subunit 2 (TfIIA-S)

Recombinant Drosophila melanogaster Transcription initiation factor IIA subunit 2 (TfIIA-S)

  • 货号：
    CSB-YP010000DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010000DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010000DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010000DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010000DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TfIIA-S
  • Uniprot No.：
  • 别名：
    TfIIA-S; CG5163; Transcription initiation factor IIA subunit 2; General transcription factor IIA subunit 2; TFIIA p14 subunit; TFIIA-14; Transcription initiation factor IIA gamma chain; TFIIA-gamma; dTFIIA-S
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-106
  • 氨基酸序列
    MSYQLYRNTT LGNTLQESLD ELIQYGQITP GLAFKVLLQF DKSINNALNQ RVKARVTFKA GKLNTYRFCD NVWTLMLNDV EFREVHEIVK VDKVKIVACD GKSGEF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    TFIIA is a component of the transcription machinery of RNA polymerase II and plays an important role in transcriptional activation. TFIIA in a complex with TBP mediates transcriptional activity. Part of a rhi-dependent transcription machinery that enables the generation of piRNA precursors from heterochromatin while maintaining the suppression of transposon-encoded promoters and enhancers. Forms a complex with Moonshiner/CG12721 and Trf2 which recruit transcriptional machinery to heterochromatin to initiate the bidirectional transcription of piRNA clusters, by interacting with the RDC (rhi, del and cuff) complex that binds to repressive H3K9me3 marks in the chromatin. This mechanism allows transcription to occur in piRNA clusters despite the lack of proper promoter elements and in the presence of the repressive H3K9me3 mark.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TFIIA subunit 2 family
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: dme:Dmel_CG5163

    STRING: 7227.FBpp0083912

    UniGene: Dm.20591